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> Mouse Monoclonal Anti-Keratin K20, lyophilized, purified [Clone IT-Ks20.10]

Mouse Monoclonal Anti-Keratin K20, lyophilized, purified [Clone IT-Ks20.10]

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Cat# 61054

Size : 50µg

Brand : Progen

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Phone : +1 850 650 7790

anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified

anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
Product: anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
Art.No.: 61054

Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Human, Mouse, Pig
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone IT-Ks20.10
Immunogen Electrophoretically purified keratin K20 from human intestinal mucosa
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym Cytokeratin 20
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) Assay dependent

IT-Ks20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.

Polypeptide recognized: protein IT (keratin K20; Mr 46,000; formerly also designated cytokeratin 20).

Reactivity on cultured cell lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.

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