Mouse Monoclonal Anti-AAV2 (intact particle), lyophilized, purified [Clone A20]

Print

Cat# 61055

Size : 50µg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Progen

Contact local distributor :

Phone : +1 850 650 7790

To be able to use the full range of Shopware 6, we recommend activating Javascript in your browser.

Highly published

Neutralizing

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

Product: anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified
Art.No.: 61055

Quantity	50 µg
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG3
Conjugate	Unconjugated
Application	Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification	Affinity chromatography
Reactivity	AAV2, AAV2 7m8, AAV3
No reactivity	AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10, AAVrh74
Storage before reconstitution	2-8°C until indicated expiry date
Storage after reconstitution	2-8°C
Intended use	Research use only
Clone	A20
Immunogen	AAV2 capsids
Concentration	50 µg/ml after reconstitution with 1 ml dist. water
Formulation	Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Binding affinity	KD value (AAV2) = 2.6E-11 M KD value (AAV3) = <1.0E-12 M
Synonym	Adeno-associated virus 2; AAV-2

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Affinity Chromatography	Assay dependent
Dot Blot	1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA	Assay dependent
Immunoprecipitation (IP)	1:5
Neutralization Assay	EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3) - assay dependent

For characterization of different stages of infection and very useful for the analysis of the AAV2 assembly process. A20 specifically reacts with AAV2 and AAV3, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
Epitope mapping experiments (Wobus et al. 2000) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV2 capsids. The antibody is also useful for neutralizing experiments.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281-93 (2000).
Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761-1766 (2000).

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

You might also be interested by the following products:

Cat#

Description

Cond.

Price Bef. VAT