Mouse LCAT (Lecithin Cholesterol Acyltransferase) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	Lcat
Gene ID:	16816
Uniprot ID:	LCAT_MOUSE
Sample Type:	serum, plasma, tissue homogenates or other biological fluids.

Tissue Specificity	Detected in blood plasma. Produced and secreted by astrocytes (at protein level). Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia.
Post Translational Modifications
Function	Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters.
Protein Name	Phosphatidylcholine-Sterol Acyltransferase 1-Alkyl-2-Acetylglycerophosphocholine Esterase Lecithin-Cholesterol Acyltransferase Phospholipid-Cholesterol Acyltransferase Platelet-Activating Factor Acetylhydrolase Paf Acetylhydrolase
Database Links	Reactome: R-MMU-8964058
Cellular Localisation	Secreted Secreted Into Blood Plasma Produced In Astrocytes And Secreted Into Cerebral Spinal Fluid (Csf)
Alternative ELISA Names	Phosphatidylcholine-Sterol Acyltransferase ELISA kit 1-Alkyl-2-Acetylglycerophosphocholine Esterase ELISA kit Lecithin-Cholesterol Acyltransferase ELISA kit Phospholipid-Cholesterol Acyltransferase ELISA kit Platelet-Activating Factor Acetylhydrolase ELISA kit Paf Acetylhydrolase ELISA kit Lcat ELISA kit
output

Information sourced from Uniprot.org

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