Mouse CD90.2/Thy-1.2 Antibody : PE
Cat# OASB00545
Size : 0.1mg
Brand : Aviva Systems Biology
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Mouse CD90.2/Thy-1.2 Antibody : PE (OASB00545)
| Datasheets/Manuals | Printable datasheet for Mouse CD90.2/Thy-1.2 Antibody : PE (OASB00545) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 30-H12 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | R-PE |
| Application | FC |
| Additional Information | Description: CD90 (Thy-1) is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily consisting of a single V-set domain. The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse thymus or spleen |
| Concentration | 0.1 mg/mL |
| Specificity | CD90.2 |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent. Representative flow data are included with this productinsert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Fluorescein (FITC) conjugate Biotin (BIOT) conjugate R-phycoerythrin (R-PE) conjugate Spectral Red conjugate Allophycocyanin (APC) conjugate <=1 ug/106 cells <=1 ug/106 cells <=1 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells |
| Application Info | Flow cytometry 4, 5, Immunohistochemistry (frozen sections) 4, In vivo and in vitro depletion of T cells 4,6,7 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 0.3 Ug/106 cells BALB/c splenocytes were double-stained with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Homans, S.W., et al. 1988. Nature 333:269 2. William, A.F., and J. Gagnon. 1982. Science 216:696 3. Williams, A.F. 1989. In: Cell Surface Antigen Thy- 1 (A.E. Reif and M. Schlesinger, eds) Marcel Dekker, New York, pp. 49- 69 4. Ledbetter, J.A., et al. 1980. J. Exp. Med. 152:280 5. Ledbetter, J.A. , and L.A. Herzenberg. 1979. Immunol. Rev. 47:63 6. Hathcock, K.S. 1991. In: Current Protocols in Immunology (J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. . .. Strober, eds) John Wiley & Sons, New York, NY, pp. 3.4.1- 3.4.3 7. Kruisbeek, A.M. 1991. In: Current Protocols in Immunology (J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. .. . … Strober, eds) John Wiley & Sons, New York, NY, pp. 4.1.1- 4.1.5. |
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| Gene Symbol | THY1 |
| Gene Full Name | Thy-1 membrane glycoprotein |
| Alias Symbols | T25, CD90, Thy-1, Thy1.1, Thy1.2, Thy-1.2 |
| NCBI Gene Id | 21838 |
| Protein Name | thy-1 membrane glycoprotein |
| Description of Target | This gene encodes a glycoprotein that is anchored to the cell surface of thymocytes, neuronal and other cells through a glycosyl-phosphatidylinositol moiety. A soluble form of the encoded protein has also been detected in serum and cerebrospinal fluid. The encoded protein undergoes further processing to generate the mature protein which mediates cell-cell interactions to trigger downstream signaling pathways. |
| Uniprot ID | P01831 |
| Protein Accession # | NP_033408.1 |
| Nucleotide Accession # | NM_009382.3 |
