Mouse CD5L (CD5 Antigen Like Protein) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	Cd5l
Gene ID:	11801
Uniprot ID:	CD5L_MOUSE
Sample Type:	serum, plasma or other biological fluids.

Tissue Specificity	Specifically expressed in tissue macrophages. Expressed in thymus, liver, spleen and lymph nodes. Present in Th17 cells.mainly present in non-pathogenic Th17 cells.
Post Translational Modifications	N-glycosylated. N-glycan at Asn-99 possesses only alpha2,6-sialylated terminals, while Asn-229 possesses both alpha2,6-sialylated and non-sialylated terminals. N-glycosylation increases secretion.
Function	Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies. Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis. Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy.
Protein Name	Cd5 Antigen-Like Apoptosis Inhibitor Expressed By Macrophages Maim Apoptosis Inhibitory 6 Sp-Alpha
Database Links
Cellular Localisation	Secreted Cytoplasm Secreted By Macrophages And Circulates In The Blood Transported In The Cytoplasm Via Cd36-Mediated Endocytosis
Alternative ELISA Names	Cd5 Antigen-Like ELISA kit Apoptosis Inhibitor Expressed By Macrophages ELISA kit Maim ELISA kit Apoptosis Inhibitory 6 ELISA kit Sp-Alpha ELISA kit Cd5l ELISA kit Aim ELISA kit Api6 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance