Mouse BNP (Brain Natriuretic Peptide) Competitive ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	2.5h
Detection:	Colormetric

Gene Symbol:	Nppb
Gene ID:	18158
Uniprot ID:	ANFB_MOUSE
Specificity:	This kit recognizes Mouse BNP in samples. No significant cross-reactivity or interference between Mouse BNP and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	The precursor molecule is proteolytically cleaved by the endoprotease Furin to produce brain natriuretic peptide 45. May undergo further proteolytic cleavage by various proteases such as DPP4, MME and possibly FAP, to give rise to a variety of shorter peptides. May be cleaved at Ser-91 by the prolyl endopeptidase FAP (seprase) activity (in vitro). May be degraded by IDE. During IDE degradation, the resulting products initially increase the activation of NPR1 and can also stimulate NPR2 to produce cGMP before the fragments are completely degraded and inactivated by IDE (in vitro).
Function	Brain natriuretic peptide 45: Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis.
Protein Name	Natriuretic Peptides B Brain Natriuretic Factor Prohormone Preprobnp Probnp Gamma-Brain Natriuretic Peptide Iso-Anp Cleaved Into - Brain Natriuretic Peptide 45 5 Kda Cardiac Natriuretic Peptide Bnp(77-121 Brain Natriuretic Peptide Bnp
Cellular Localisation	Brain Natriuretic Peptide 45: Secreted Detected In Blood
Alternative ELISA Names	Natriuretic Peptides B ELISA kit Brain Natriuretic Factor Prohormone ELISA kit Preprobnp ELISA kit Probnp ELISA kit Gamma-Brain Natriuretic Peptide ELISA kit Iso-Anp Cleaved Into - Brain Natriuretic Peptide 45 ELISA kit 5 Kda Cardiac Natriuretic Peptide ELISA kit Bnp(77-121 ELISA kit Brain Natriuretic Peptide ELISA kit Bnp ELISA kit Nppb ELISA kit
Specificity	This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-100	95
EDTA plasma(n=8)	95-108	100
Cell culture media(n=8)	91-105	98

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	51.40	130.40	492.60	52.70	130.30	444.70
Standard deviation	3.20	7.20	22.70	2.70	7.80	17.30
CV (%)	6.23	5.52	4.61	5.12	5.99	3.89

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance