Mouse ATP4a (ATPase, H+/K+ Exchanging Alpha Polypeptide) Sandwich ELISA Kit Ready-To-Use

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	Atp4a
Gene ID:	11944
Uniprot ID:	ATP4A_MOUSE
Immunogen Region:	Ready-To-Use
Sample Type:	tissue homogenates or other biological fluids.

Tissue Specificity	Expressed in parietal cells (at protein level).
Post Translational Modifications
Function	The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state.
Protein Name	Potassium-Transporting Atpase Alpha Chain 1 Gastric H(+/K(+ Atpase Subunit Alpha Proton Pump
Database Links	Reactome: R-MMU-936837
Cellular Localisation	Apical Cell Membrane Multi-Pass Membrane Protein Localized In The Apical Canalicular Membrane Of Parietal Cells
Alternative ELISA Names	Potassium-Transporting Atpase Alpha Chain 1 ELISA kit Gastric H(+/K(+ Atpase Subunit Alpha ELISA kit Proton Pump ELISA kit Atp4a ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance