Mouse ATG7 (Autophagy Related Protein 7) Sandwich ELISA Kit Ready-To-Use

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	Atg7
Gene ID:	74244
Uniprot ID:	ATG7_MOUSE
Immunogen Region:	Ready-To-Use
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Post Translational Modifications	Acetylated by EP300.
Function	E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner.
Protein Name	Ubiquitin-Like Modifier-Activating Enzyme Atg7 Atg12-Activating Enzyme E1 Atg7 Autophagy-Related Protein 7 Apg7-Like Magp7 Ubiquitin-Activating Enzyme E1-Like Protein
Database Links	Reactome: R-MMU-1632852 Reactome: -MMU-6798695 Reactome: -MMU-983168
Cellular Localisation	Cytoplasm Preautophagosomal Structure Localizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme
Alternative ELISA Names	Ubiquitin-Like Modifier-Activating Enzyme Atg7 ELISA kit Atg12-Activating Enzyme E1 Atg7 ELISA kit Autophagy-Related Protein 7 ELISA kit Apg7-Like ELISA kit Magp7 ELISA kit Ubiquitin-Activating Enzyme E1-Like Protein ELISA kit Atg7 ELISA kit Apg7l ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance