Mouse Anti-FLAG Antibody
Cat# OAPA00047
Size : 0.5mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Mouse Anti-FLAG Antibody (OAPA00047)
| Datasheets/Manuals | Printable datasheet for Mouse Anti-FLAG Antibody (OAPA00047) |
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| Product Format | Lyophilized PBS Physical Appearance: Sterile filtered white freeze-dried powder |
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| Clonality | Polyclonal |
| Clone | NYRFLAG |
| Isotype | IgG1, kappa. |
| Host | Mouse |
| Reconstitution and Storage | Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. In lyophilized form, for long periods, store at 4C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20C. |
| Immunogen | Flag-conjugated proteins. |
| Purification | Protein A & Ion exchange column. |
| Specificity | Will recognize FLAG (DYKDDDDK) conjugated at either the amino or carboxy terminus. Can be used bound to various resins for affinity purifications. |
| Source | BALB/c mice |
| Application Info | The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins. Titer: By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Gene Symbol | RGS3 |
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| Description of Target | A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins. |
