Reagents and instruments for immunology, cell biology and molecular biology.
 
> Milenia® GenLine HybriDetect

Milenia® GenLine HybriDetect

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Cat# MGHD1

Size : 100tests

Brand : Milenia Biotec

Contact local distributor :


Phone : +1 850 650 7790

( excludes VAT and shipping costs)
100

The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons

Safety Data Sheet
Overview
Name of Product
HybriDetect - Universal Lateral Flow Assay Kit
Catalog Number
MGHD 1
Short Info

The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons

Method/Platform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
5 pg DNA, eqivalent to agarose gel electrophoresis
Test Principle

HybriDetect is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for a wide range of analytes such as antibodies, gene amplification products or proteins . The results can be interpreted qualitative or quantitative.

 

 

Documents
Protocol
Brief Instructions

Click here for the Instructions for Use

Storage

2-8°C

Components

HybriDetect dipsticks, HybriDetect assay buffer

References
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  • Combining CRISPR-Cas12a with Terminal Deoxynucleotidyl Transferase dependent reporter elongation for pathogen detection using Lateral Flow Test Strips (2022)
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  • Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay output (2022)
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  • CRISPR-based diagnostics detects invasive insect pests (2023)
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  • CRISPR-based, genotype-specific detection of yellow head virus genotype 1 with fluorescent, lateral flow and DNAzyme-assisted colorimetric readouts (2023)
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  • CRISPR-Cas fluorescent cleavage assay coupled with recombinase polymerase amplification for sensitive and specific detection of Enterocytozoon hepatopenaei. (2020)
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  • CRISPR-Cas12-based detection of SARS-CoV-2. (2020)
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  • CRISPR-Cas12a-Based Diagnostic Method for Japanese Encephalitis Virus Genotypes I, III, and V (2023)
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  • CRISPR-Cas13a-Based Assay for Accurate Detection of OXA-48 and GES Carbapenemases (2023)
    Ortiz-Cartagena, C., Pablo-Marcos, D., Fernández-García, L., Blasco, L., Pacios, O., Bleriot, I., Siller, M., López, M., Fernández, J., Aracil, B., Fraile-Ribot, P. A., García-Fernández, S., Fernández-Cuenca, F., Hernández-García, M., Cantón, R., Calvo-Montes, J., & Tomás, M.
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  • CRISPR-ENHANCE: An enhanced nucleic acid detection platform using Cas12a (2021)
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  • CRISPR/Cas12-Based Ultra-Sensitive and Specific Point-of-Care Detection of HBV (2021)
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  • CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus (2023)
    Bhardwaj, P., Nanaware, N. S., Behera, S. P., Kulkarni, S., Deval, H., Kumar, R., Dwivedi, G. R., Kant, R., & Singh, R.
  • CRISPR/Cas12a-Based Ultrasensitive and Rapid Detection of JAK2 V617F Somatic Mutation in Myeloproliferative Neoplasms (2021)
    Chen, M., Zhang, C., Hu, Z., Li, Z., Li, M., Wu, L., Zhou, M., & Liang, D.
  • CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics (2021)
    Osborn, M. J., Bhardwaj, A., Bingea, S. P., Knipping, F., Feser, C. J., Lees, C. J., Collins, D. P., Steer, C. J., Blazar, B. R., & Tolar, J.
  • CRISPRs in the human genome are differentially expressed between malignant and normal adjacent to tumor tissue (2022)
    van Riet, J., Saha, C., Strepis, N., Brouwer, R. W. W., Martens-Uzunova, E. S., van de Geer, W. S., Swagemakers, S. M. A., Stubbs, A., Halimi, Y., Voogd, S., Tanmoy, A. M., Komor, M. A., Hoogstrate, Y., Janssen, B., Fijneman, R. J. A., Niknafs, Y. S., Chinnaiyan, A. M., van IJcken, W. F. J., van der Spek, P. J. Louwen, R.
  • Detection assay for HPV16 and HPV18 by loop‑mediated isothermal amplification with lateral flow dipstick (2017)
    Kumvongpin R, Jearanaikoon P, Wilailuckana C, Sae‑Ung N, Prasongdee P, Daduang S, Wongsena M, Boonsiri P, Kiatpathomchai W, Swangvaree SS, Sandee A, Daduang J
  • Detection of a single nucleotide polymorphism for insecticide resistance using recombinase polymerase amplification and lateral flow dipstick detection (2022)
    Ahmed, M., Pollak, N. M., Devine, G. J., & Macdonald, J.
  • Detection of Cryptosporidium Species in Kenya Using Lateral Flow Loop-Mediated Isothermal Amplification (2020)
    Mamba T. S.
  • Detection of Entamoeba histolytica by Recombinase Polymerase Amplification. Am. J. Trop. Med. 2015; 93 (3) 591-595 (2015)
    Nair G. et al.
  • Detection of Helminth Ova in Wastewater Using Recombinase Polymerase Amplification Coupled to Lateral Flow Strips. (2020)
    Ravindran V. B., Khallaf B., Surapaneni A., Crosbie N.D., Soni S.K., Ball A.S.
  • Detection of Infectious Viruses Using CRISPR-Cas12-Based Assay (2021)
    Talwar, C. S., Park, K. H., Ahn, W. C., Kim, Y. S., Kwon, O. S., Yong, D., Kang, T., & Woo, E.
  • Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples. Biomed. Res. Int. 2013; 1-6 (2013)
    Kaewphinit T., Arunrut N., Kiatpathomchai W., Santiwatanakul S., Jaratsing P., Chansiri K.
  • Detection of Reverse Transcriptase LAMP-Amplified Nucleic Acid from Oropharyngeal Viral Swab Samples Using Biotinylated DNA Probes through a Lateral Flow Assay (2023)
    Agarwal, S., Hamidizadeh, M., & Bier, F. F.
  • Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay (2022)
    Zasada, A. A., Mosiej, E., Prygiel, M., Polak, M., Wdowiak, K., Formińska, K., Ziółkowski, R., Żukowski, K., Marchlewicz, K., Nowiński, A., Nowińska, J., Rastawicki, W., & Malinowska, E.
  • Detection of Skeletonema costatum based on loop-mediated isothermal amplification combined with lateral flow dipstick. Mol Cell Probes. 2017 Dec;36:36-42. (2017)
    Huang H. L. et al.
  • Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification (2023)
    Husseini, A. A., Yazdani, A. M., Ghadiri, F., & Şişman, A.
  • Development and application of sensitive, specific, and rapid CRISPR-Cas13-based diagnosis (2021)
    Shihong Gao, D., Zhu, X., & Lu, B.
  • Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of Monkeypox Virus (2022)
    Mao, L., Ying, J., Selekon, B., Gonofio, E., Wang, X., Nakoune, E., Wong, G., & Berthet, N.
  • Development and Clinical Validation of RT-LAMP-Based Lateral-Flow Devices and Electrochemical Sensor for Detecting Multigene Targets in SARS-CoV-2 (2022)
    Canuti, M., Saxena, A., Rai, P., Mehrotra, S., Baby, S., Singh, S., Srivastava, V., Priya, S., & Sharma, S. K.
  • Development and evaluation of a novel visual and rapid detection assay for toxigenic Fusarium graminearum in maize based on recombinase polymerase amplification and lateral flow analysis (2022)
    Liang, X., Zhang, X., Haseeb, H. A., Tang, T., Shan, J., Yin, B., & Guo, W.
  • Development and evaluation of an isothermal recombinase polymerase amplification–lateral flow assay for rapid detection of strawberry vein banding virus in the field (2022)
    Zou, X., Yuan, S., Dong, C., & Gao, Q.
  • Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1 (2018)
    Wang H, Hou P, Zhao G, Yu L, Gao YW, He H
  • Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings (2023)
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    Lee, H. J., Cho, I. S., Ju, H. J., & Jeong, R. D.
  • Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay (2019)
    Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L,Jia WZ, Fu BQ
  • Rapid and visual detection of Verticillium dahliae using recombinase polymerase amplification combined with lateral flow dipstick. (2020)
    Ju Y., Li C., Shen P., et. al.,
  • Rapid authentication of Cordyceps by lateral flow dipstick. J. Pharm. Biomed. Anal 2015, 111, 306-310 (2015)
    Wong YL et al.
  • Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus-3 in koi and common carp. J Fish Dis. 2018 May;41(5):761-772. (2018)
    Soliman H. and El-Matoubli M.
  • Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay. (2020)
    Broughton J. P., Deng X., Fasching C.L., Singh J., Streithorst J., Granados, A., Sotomayor-Gonzalez A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C., Guevara H., Wadford D., Chen J., Chio C.Y.
  • Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics. (2020)
    Lu L., Li F., Chen Q., et. al
  • Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk (2018)
    Hou P, Zhao G, Wang H, He C, He H
  • Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay (2019)
    Peng Y, Zheng X, Kann B, Li W, Zhang W, Jiang T, Lu J, Qin A
  • Rapid detection of Edwardsiella ictaluri in yellow catfish (Pelteobagrus fulvidraco) by real-time RPA and RPA-LFD (2022)
    Li, H., Zhang, L., Yu, Y., Ai, T., Zhang, Y., & Su, J.
  • Rapid detection of Enterococcus and vancomycin resistance using recombinase polymerase amplification (2021)
    Panpru, P., Srisrattakarn, A., Panthasri, N., Tippayawat, P., Chanawong, A., Tavichakorntrakool, R., Daduang, J., Wonglakorn, L., & Lulitanond, A.
  • Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification (2013)
    Boyle, D. S., Lehman, D. A., Lillis, L., Peterson, D., Singhal, M., Armes, N., Parker, M., Piepenburg, O., & Overbaugh, J.
  • Rapid detection of kdr mutation F1534C in Aedes aegypti using recombinase polymerase amplification and lateral flow dipsticks (2022)
    Ahmed, M., Nath, N. S., Hugo, L. E., Devine, G. J., Macdonald, J., & Pollak, N. M.
  • Rapid detection of mcr-1 by recombinase polymerase amplification (2018)
    Xu, J., Wang, X., Yang, L., Kan, B., & Lu, X.
  • Rapid detection of mcyG gene of microcystins producing cyanobacteria in water samples by recombinase polymerase amplification combined with lateral flow strips (2021)
    Li, J., Feng, M., & Yu, X.
  • Rapid detection of methicillin-resistant Staphylococcus aureus in positive blood-cultures by recombinase polymerase amplification combined with lateral flow strip (2022)
    Srisrattakarn, A., Panpru, P., Tippayawat, P., Chanawong, A., Tavichakorntrakool, R., Daduang, J., Wonglakorn, L., & Id, A. L.
  • Rapid detection of monkeypox virus using a CRISPR-Cas12a mediated assay: a laboratory validation and evaluation study (2023)
    Caly, L., Pasricha, S., Williamson, D. A., Kerry MBiotech, W. J., Whitehead, L. W., Williams, L., Cooney, J. P., Pellegrini, M., Savic, I., Prestedge, J., Tran, T., Lim, C. K., Towns, J. M., Bradshaw, C. S., Fairley, C., F Chow, E. P., Chen, M. Y., Jen Low, S., O, M. T., … Williamson, D. A.
  • Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion–Insertion Mutation in S-Protein Gene (2023)
    Tanuri, A., Malaga, J. L., Pajuelo, M. J., Okamoto, M., Kagning Tsinda, E., Otani, K., Tsukayama, P., Mascaro, L., Cuicapuza, D., Katsumi, M., Kawamura, K., Nishimura, H., Sakagami, A., Ueki, Y., Omiya, S., Okamoto, S., Nakayama, A., Fujimaki, S., Yu, C., … Saito, M.
  • Rapid Detection of Strawberry Mild Yellow Edge Virus with a Lateral Flow Strip Reverse Transcription Recombinase Polymerase Amplification Assay (2022)
    Zou, X., Dong, C., Ni, Y., & Gao, Q.
  • Rapid detection of strawberry mottle virus using reverse transcription recombinase polymerase amplification with lateral flow strip (2022)
    Zou, X., Dong, C., Ni, Y., Yuan, S., & Gao, Q. H.
  • Rapid detection of the rice false smut fungus Ustilaginoidea virens by lateral flow strip-based recombinase polymerase amplification assay (2023)
    Jia-cheng, X., San-lian, W., Yue, L., Yuan-di, X., Jing, Y., Ting, Z., Jia-jia, C., Zheng-guang, Z., Dan-yu, S., & Hai-feng, Z.
  • Rapid Detection of the Varicella-Zoster Virus Using a Recombinase-Aided Amplification-Lateral Flow System (2022)
    Bienes, K. M., Mao, L., Selekon, B., Gonofio, E., Nakoune, E., Wong, G., & Berthet, N.
  • Rapid Diagnosis and Visual Detection of Potato Cyst Nematode (Globodera rostochiensis) Using Recombinase Polymerase Amplification Combination with Lateral Flow Assay Method (RPA-LFA) (2022)
    Wang, X. ;, Lei, R. ;, Peng, H. ;, Jiang, R. ;, Shao, H. D. ;, Peng, J. J. ;, Wang, X., Lei, R., Peng, H., Jiang, R., Shao, H., Ge, J., & Peng, D.
  • Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay. (2020)
    Seo H., Kil E., Fadhila C., et. al.
  • Rapid Diagnostic for the Detection of the Four Dengue Virus Serotypes in Clinically Relevant Matrices (2023)
    Pollak, N. M., Olsson, M., Ahmed, M., Tan, J., Lim, G., Setoh, Y. X., Wong, J. C. C., Lai, Y. L., Hobson-Peters, J., Macdonald, J., & McMillan, D.
  • Rapid DNA extraction and colorimetric amplicon visualisation speed up LAMP-based detection of soybean allergen in foods (2023)
    Schäfer, L., Allgöwer, S., & Holzhauser, T.
  • Rapid isothermal point-of-care test for screening of SARS-CoV-2 (2023)
    Zingg, J.-M., Yang, Y.-P., Seely, S., Joshi, P., Roshid, M. H. O., Iribarren Latasa, F., O’Connor, G., Alfaro, J., Riquelme, E., Bernales, S., Dikici, E., Deo, S., & Daunert, S.
  • Rapid molecular assays for the detection of the four dengue viruses in infected mosquitoes (2022)
    Ahmed, M., Pollak, N. M., Hugo, L. E., van den Hurk, A. F., Hobson-Peters, J., Macdonald, J., & Paradkar, P. N.
  • Rapid molecular detection of CMY-2, and CTX-M group 1 and 9 variants via recombinase polymerase amplification (2023)
    Ertl, N. G., Irwin, A. D., Macdonald, J., Bauer, M. J., Wang, C. Y. T., Harris, P. N. A., Heney, C., Zowawi, H. M., & Whiley, D. M.
  • Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (2023)
    Greeshma, M., Bhat, A. I., & Jeevalatha, A.
  • Rapid PCR-lateral flow assay for the onsite detection of Atlantic white shrimp (2023)
    Kwawukume, S., Velez, F. J., Williams, D., Cui, L., & Singh, P.
  • Rapid sample preparation and low-resource molecular detection of hepatopancreatic parvoviruses (HPV) by recombinase polymerase amplification lateral flow detection assay in shrimps (Fenneropenaeus merguiensis). (2022)
    Pollak ID, N. M., Fais, O., Kristoffersen, J., Phuthaworn, C., Knibb, W., & Macdonald, J. I.
  • Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay (2020)
    Schermer B., Fabretti F., Damagnez M., et. al.
  • Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network (2022)
    Xie, S., Tao, D., Fu, Y., Xu, B., Tang, Y., Steinaa, L., Hemmink, J. D., Pan, W., Huang, X., Nie, X., Zhao, C., Ruan, J., Zhang, Y., Han, J., Fu, L., Ma, Y., Li, X., Liu, X., & Zhao, S.
  • Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
    Wang H, Sun M, Xu D, Podok P, Xie J, Jiang Y, Lu L
  • Rapid visual detection of Enterocytozoon hepatopenaei by recombinase polymerase amplification combined with a lateral flow dipstick (2022)
    Pang, J., Zhao, C., Liu, Z., Lu, Q., He, X., Weng, S., & Jianguo
  • Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
    Zhao G, Wang H, Hou P, He C, He H
  • Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test (2022)
    Qi, Y., Li, W., Li, X., Shen, W., Lv, R., Lu, N., Li, J., Zhuang, S., Xu, Y., Gui, Q., Shen, H., & Li, Y.
  • Rapid Visual Detection of Streptococcus suis and Actinobacillus pleuropneumoniae Through Duplex Recombinase Polymerase Amplification Combined with Lateral Flow Dipsticks (2022)
    Zhang, S., Xie, H., Liu, M., Zheng, A., Yan, H., Duan, M., Wei, X., Teng, Z., Zhang, H., & Xia, X.
  • Rapid visual nucleic acid detection of Vibrio alginolyticus by recombinase polymerase amplification combined with CRISPR/Cas13a. (2023)
    Wang, Y., Hou, Y., Liu, X., Lin, N., Dong, Y., Liu, F., Xia, W., Zhao, Y., Xing, W., Chen, J., & Chen, C.
  • Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO (2022)
    Casati, B., Verdi, J. P., Hempelmann, A., Kittel, M., Klaebisch, A. G., Meister, B., Welker, S., Asthana, S., Giorgio, S. di, Boskovic, P., Man, K. H., Schopp, M., Ginno, P. A., Radlwimmer, B., Stebbins, C. E., Miethke, T., Papavasiliou, F. N., & Pecori, R.
  • Rapid, CRISPR-based, field-deployable detection of white spot syndrome virus in shrimp (2019)
    Sullivan TJ, Arun KD, Cruz-Flores R, Bodnar AG
  • Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR (2021)
    Brandsma, E., Verhagen, H., van de Laar, T., Claas, E., Cornelissen, M., & van den Akker, E.
  • Rapid, sensitive, and specific, low-resource molecular detection of Hendra virus (2023)
    Pollak, N. M., Marsh, G. A., Olsson, M., McMillan, D., & Macdonald, J.
  • Rational Programming of Cas12a for Early-Stage Detection of COVID-19 by Lateral Flow Assay and Portable Real-Time Fluorescence Readout Facilities (2022)
    Yi, Z., de Dieu Habimana, J., Mukama, O., Li, Z., Odiwuor, N., Jing, H., Nie, C., Hu, M., Lin, Z., Wei, H., & Zeng, L.
  • Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density. (2020)
    Lalremruata A., Nguyen T., MCCall M., et. al.
  • Recombinase polymerase amplification assay combined with a dipstick-readout for rapid detection of Mycoplasma ovipneumoniae infections (2021)
    Gupta, S. K., Deng, Q., Gupta, T. B., Maclean, P., Jores, J., Heiser, A.
  • Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. Parasit Vectors. 2018 Apr 11;11(1):234. (2018)
    Soliman H. et al.
  • Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome (2014)
    Jaroenram, W., & Owens, L.
  • Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Rapid Detection of OXA-48-like Carbapenemase Genes in Enterobacterales (2022)
    Hemwaranon, P., Srisrattakarn, A., Lulitanond, A., Tippayawat, P., Tavichakorntrakool, R., Wonglakorn, L., Daduang, J., & Chanawong, A.
  • Recombinase polymerase amplification lateral flow dipstick (RPA-LF) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) (2021)
    An, X., Zhao, Y., Cui, J., Liu, Q., Yu, L., Zhan, X., Zhang, W., He, L., & Zhao, J.
  • Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections (2021)
    Nie, Z., Zhao, Y., Shu, X., Li, D., Ao, Y., Li, M., Wang, S., Cui, J., An, X., Zhan, X., He, L., Liu, Q., & Zhao, J.
  • Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters. (2020)
    Jiang W., Ren Y., Han X., Xue J., Shan T., Chen Z., Liu Y., Quan W.
  • Recombinase Polymerase Amplification/Cas12a-Based Identification of Xanthomonas arboricola pv. pruni on Peach (2021)
    Luo, M., Meng, F. Z., Tan, Q., Yin, W. X., & Luo, C. X.
  • REVEALR: A Multicomponent XNAzyme-Based Nucleic Acid Detection System for SARS-CoV-2 (2021)
    Yang, K., & Chaput, J. C.
  • Reverse transcription loop-mediated isothermal amplification combined with lateral flow device (RT-LAMP-LFD) for swine influenza virus detection (2021)
    Asih, A. U., Janetanakit, T., Nasamran, C., Bunpapong, N., Boonyapisitsopa, S., Amonsin, A.
  • Reverse Transcription-Loop-Mediated Isothermal Amplification-CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2 (2022)
    Ortiz-Cartagena, C., Fernández-García, L., Blasco, L., Pacios, O., Bleriot, I., López, M., Cantón, R., & Tomás, M.
  • RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern (2023)
    Tang, G., Zhang, Z., Tan, W., Long, F., Sun, J., Li, Y., Zou, S., Yang, Y., Cai, K., Li, S., Wang, Z., Liu, J., Mao, G., Ma, Y., Zhao, G.-P., Tian, Z.-G., & Zhao, W.
  • Sensitive and Easy-Read CRISPR Strip for COVID-19 Rapid Point-of-Care Testing (2021)
    Li, H., Dong, X., Wang, Y., Yang, L., Cai, K., Zhang, X., Kou, Z., He, L., Sun, S., Li, T., Nie, Y., Li, X., & Sun, Y.
  • Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD) (2022)
    Onchan, W., Ritbamrung, O., Changtor, P., Pradit, W., Chomdej, S., Nganvongpanit, K., Siengdee, P., Suyasunanont, U., & Buddhachat, K
  • Sensitive and Rapid Detection of Citrus Scab Using an RPA-CRISPR/Cas12a System Combined with a Lateral Flow Assay (2021)
    Shin, K., Kwon, S. H., Lee, S. C., & Moon, Y. E.
  • Sensitive and Specific Exonuclease III-Assisted Recombinase-Aided Amplification Colorimetric Assay for Rapid Detection of Nucleic Acids (2023)
    Zhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, L., Hemmink, J. D., Han, J., Li, X., Xu, J., Zhao, S., Xie, S., & Zhao, C.
  • Sensitive naked-eye detection of telomerase activity based on exponential amplification reaction and lateral flow assay (2022)
    Cheng, X. R., Wang, F., Liu, C. yun, Li, J., Shan, C., Wang, K., Wang, Y., Li, P. F., & Li, X. M
  • Sequence-Specific Detection of ORF1a, BRAF, and ompW DNA Sequences with Loop Mediated Isothermal Amplification on Lateral Flow Immunoassay Strips Enabled by Molecular Beacons (2021)
    Varona, M., Eitzmann, D. R., & Anderson, J. L.
  • SHERLOCK: nucleic acid detection with CRISPR nucleases (2019)
    Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F
  • SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African Trypanosomiasis (2022)
    Sima, N., Dujeancourt-Henry, A., Perlaza, B. L., Ungeheuer, M.-N., Rotureau, B., & Glover, L.
  • Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants (2022)
    Arizti-Sanz, J., Bradley, A., Zhang, Y. B., Boehm, C. K., Freije, C. A., Grunberg, M. E., Kosoko-Thoroddsen, T.-S. F., Welch, N. L., Pillai, P. P., Mantena, S., Kim, G., Uwanibe, J. N., John, O. G., Eromon, P. E., Kocher, G., Gross, R., Lee, J. S., Hensley, L. E., MacInnis, B. L., Myhrvold, C.
  • Specific Detection of Influenza A and B Viruses by CRISPR-Cas12a-Based Assay (2021)
    Park, B. J., Park, M. S., Lee, J. M., & Song, Y. J.
  • Supporting Information Clustered Regularly Interspaced Short Palindromic Repeats / Cas9- Mediated Lateral Flow Nucleic Acid Assay. (2020)
    Wang X., Xion E., Tian T., Cheng M., Lin W., Wang H.
  • The development of highly specific and sensitive primers for the detection of potentially allergenic soybean (Glycine max) using loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD). (2020)
    Allgöwer S., Hartmann C., Holzhauser T.
  • The Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick for Detection of Karlodinium veneficum. Harmful Algae 2017; 62, 20-29. (2017)
    Huang HL et al.
  • Time course of detection of human male DNA from stained blood sample on various surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction (2018)
    Kanchanaphum P
  • Toward a CRISPR-Based Point-of-Care Test for Tomato Brown Rugose Fruit Virus Detection (2022)
    Bernabé-Orts, J. M., Hernando, Y., & Aranda, M. A.
  • Trends and Innovations in Biosensors for COVID-19 Mass Testing (2020)
    Santiago I.
  • Ultrasensitive ImmunoMag-CRISPR Lateral Flow Assay for Point-of-Care Testing of Urinary Biomarkers (2023)
    Lee, I., Kwon, S.-J., Heeger, P., & Dordick, J. S.
  • Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida (2019)
    Zhao G, He H, Wang H
  • Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus. Virusdisease. 2015 Sep; 26(3):189-95 (2015)
    Kusumawati A. et al.
  • Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis (2019)
    Wu L, Ye L, Wang Z, Cui Y, Wang J
  • Variety Differentiation: Development of a CRISPR DETECTR Method for the Detection of Single Nucleotide Polymorphisms (SNPs) in Cacao (Theobroma cacao) and Almonds (Prunus dulcis) (2023)
    Wax, N., La-Rostami, F., Albert, C., & Fischer, M.
  • Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2 (2021)
    Marsic, T., Ali, Z., Tehseen, M., Mahas, A., Hamdan, S., & Mahfouz, M.
  • Visual Detection of Canine Parvovirus Based on Loop-Mediated Isothermal Amplification Combined with Enzyme-Linked Immunosorbent Assay and with Lateral Flow Dipstick. J. Vet. Med. Sci. 2014 (2014)
    Sun YL et al.
  • Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR-Cas13a. (2020)
    Chang Y., Deng Y., Li T.
Videos

FAQs
A:

Yes, but initially negative strips may a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.

A:

HybriDetect allows the detection of 1 target on the strip, while HybriDetect 2T allows the detection of 2 targets on the same strip.

A:

This depends on the size of the amplicon. The shorter the amplicon is, the lower is the sensitivity of the agarose gel. If the amplicon is 100 bases long HybriDetect could possibly be 1.000 fold more sensitive compared to the agarose gel.

A:

Yes, but initially negative strips may a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.

A:

Yes, we accept credit card for payment if you purchase our products online via our webshop.

A:

Yes, you can download the pdf-file of the MSDS for HybriDetect here here and for HybriDetect 2T here.

A:

The products are in inventory and we ship the same day if we receive the order prior 12.00 CET.

A:

No, kits can be shipped at ambient temperatures, but should be stored at 2 – 8°C after receipt.

A:

Yes, the kits will still perform well. We have made stability and could demonstrate that the kits still perform well after storing them for 100 days at 37°C.

A:

The sum of the volume of the sample and the buffer should not exceed 100 µl.

A:

This phenomenon is due to the formation of primer dimers. In this event biotinylated and FITC-labeled primers bind to each other and form dimers resulting in false positive results. You need to redesign your primers in order to prevent primer dimerization.

A:

The shelf life of the kits from the time of production is 24 months. Milenia Biotec is manufacturing fresh lots twice a year, meaning that our customers usually get kits with a remaining shelf life of not less than 12 months.

A:

Yes, in case the concentration of labeled primers or probes is too high, this may lead to a decreased sensitivity of the test. In this case primer or probe concentration need to be reduced. Furthermore, components of the sample or the sample buffer, including SDS, DTT or urea may have a negative impact on the performance of the test. In this case the sample can be diluted in order to „dilute the effect out“. However this approach will result in a reduced sensitivity of the test. It is also possible to develop a modified assay buffer to reduce the interferences.

A:

Yes, we demonstrated that HybriDetect (MGHD 1) still performs well at temperatures up to 75°C and HybriDetect 2T at temperatures up to 65°C.

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