LOC100736962 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommended Dilution	Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Recommended Dilutions: Western blot: 1/1000-1/5000 Immunohistochemistry. ELISA: 1/14000. This product has been assayed against 1.0 ug of esterase [porcine liver] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested.
Reactivities	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Esterase from porcine liver
Specificity	This antibody detects porcine esterase. Cross reactivity against esterase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Esterase [porcine liver].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 397478 Pig UniProt ID Q29550
Synonyms	CES2, SES1, Liver carboxylesterase 1, HMSE, Egasyn, hBr1
Reference Data