Live-or-Dye™ 665/685 Fixable Viability Staining Kit (200 assays)
Cat# 32013
Size : 1kit
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
Live-or-Dye™ Fixable Viability Staining Kits
Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. A wide variety of dye options for standard flow, spectral flow, and fluorescence microscopy.
Product Attributes
| Apoptosis/viability marker | Dead cell stain |
|---|---|
| For live or fixed cells | Covalent & fixable stains, For live/intact cells |
| Detection method/readout | Fluorescence microscopy, Flow cytometry |
| Assay type/options | Endpoint assay |
| Fixation options | Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining |
| Colors | Blue, Green, Orange, Red, Far-red, Near-infrared |
| Storage Conditions | Store at -10 to -35 °C, Protect dye component from light, Desiccate |
Product Description
Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.
Features
- Highly dead-cell specific
- Covalent labeling with reactive dyes
- Withstand fixation and permeabilization
- Wide selection of colors
- Dye options designed for spectral flow
- Sampler kits for convenience
Single Color Kit Components
- Live-or-Dye™ fixable dead cell dye
- Anhydrous DMSO
Sampler Kit Components
- 5 different Live-or-Dye™ fixable dead cell dyes
- Anhydrous DMSO
Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.
How It Works
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeant amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Biotium has a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum (see Table 1 below), for maximal flexibility in designing multi-color flow panels.
We also offer two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Table 2 below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.
Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Table 1: Single-Color Live-or-Dye™ Kits
Cat. No. | Viability Dye | Compatible lasers (nm) | Optimal detection channels | Notes |
|---|---|---|---|---|
| 32018, 32018-T | Live-or-Dye™ 330/410 | 355, 375 | BUV395 | |
| 32002, 32002-T | Live-or-Dye™ 350/448 | 355, 375 | DAPI | |
| 32014,32014-T | Live-or-Dye™ 375/600 | 355, 375, 405 | Spectral scan, BUV615, BV605 | Developed for spectral cytometry |
| 32003, 32003-T | Live-or-Dye™ 405/452 | 405 | Pacific Blue®, BV421, BV450 | |
| 32009, 32009-T | Live-or-Dye™ 405/545 | 405 | AmCyan, BV510 | |
| 32004, 32004-T | Live-or-Dye™ 488/515 | 488 | FITC | Validated for microscopy |
| 32012, 32012-T | Live-or-Dye™ 510/550 | 488, 532 | Spectral scan | Developed for spectral cytometry |
| 32005, 32005-T | Live-or-Dye™ 568/583 | 488, 532, 561 | PE, PI | Validated for microscopy |
| 32006, 32006-T | Live-or-Dye™ 594/614 | 488, 532, 561 | PI, PE-CF®594, PE-Texas Red® | Validated for microscopy |
| 32015, 32015-T | Live-or-Dye™ 615/740 | 633-640 | Spectral scan, APC-Cy®7 | Developed for spectral cytometry |
| 32007, 32007-T | Live-or-Dye™ 640/662 | 633-640 | APC | Validated for microscopy |
| 32013, 32013-T | Live-or-Dye™ 665/685 | 633-640 | Spectral scan, AF700 | Developed for spectral cytometry |
| 32008, 32008-T | Live-or-Dye™ 750/777 | 633-640, 785 | APC-Cy®7 | The brightest dye for the APC-Cy®7 channel |
| 32011, 32011-T | Live-or-Dye™ 787/808 | 785, 808 | APC-Cy®7, IR800 |
Table 2: Live-or-Dye™ Sampler Kits
Kit Name | Catalog No. | Included dyes: Ex/Em (Cat No.) | Application |
|---|---|---|---|
| Live-or-Dye™ Fixable Viability Sampler Kit, Standard | 32016 | • 350/448 (32002A) • 405/545 (32009A) • 488/515 (32004A) • 568/583 (32005A) • 640/662 (32007A) | Designed for use on most standard flow cytometry laser and filter configurations |
| Live-or-Dye™ Fixable Viability Sampler Kit, Spectral | 32017 | • 350/448 (32002A) • 375/600 (32014A) • 510/550 (32012A) • 615/740 (32015A) • 665/685 (32013A) | Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores |
References
1. Methods (2015) 84;pp44-52 doi:10.1016/j.ymeth.2015.04.025 Live-or-Dye™ 488
2. Dev Comp Immunol (2017) Dec;77:95-105. doi: 10.1016/j.dci.2017.07.021 Live-or-Dye™ 350
3. PLoS ONE (2018) 13(12): e0208649 doi.org/10.1371/journal.pone.0208649 Live-or-Dye™ 640
3. Ann Surg Oncol (2018) 25, 3755–3763 doi:10.1245/s10434-018-6739-4 Live-or-Dye™ 750
4. MBio (2018) Aug 21;9(4) doi:10.1128/mBio.01403-18
5. Cell Physiol Biochem (2018) 49:828–836 doi:10.1159/000493110 Live-or-Dye™ 350
6. FEBS J (2019) Nov 26. doi:10.1111/febs.15156 Live-or-Dye™ 488
7. JBC (2019) 294, 16918-16929 doi:10.1074/jbc.RA119.009141 Live-or-Dye™ 488
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