Lipo3K Transfection Reagent is a cationic lipid transfection reagent widely used in cell transfection, which can transfect DNA or RNA (such as siRNA/mRNA) into various types of cells, including adherent cells and suspension cells and even some difficult-to-transfect cells. The transfection reagent is used in the same way as the commonly used Lipofectamine 3000 Reagent, and the transfection efficiency of this transfection reagent has been tested to be comparable to the Lipofectamine® 3000 Reagent.

Compared with Lipo2K, another commonly used lipid transfection reagent, this transfection reagent has higher transfection efficiency and lower cytotoxicity. Compared to Lipo2K, this transfection reagent has a 2-10 fold increase in transfection efficiency, so it is ideal for difficult-to-transfect cells. At the same time, because of the lower cytotoxicity, this reagent does not need to be changed medium after transfection, and the cells can be directly collected for detection 24-48 h after transfection, which is simpler and more convenient. Moreover, a transfection enhancement reagent (Lipo3K-A Reagent) is provided in this kit, which can help plasmid DNA enter the nucleus and greatly increase transfection efficiency. The transfection enhancement reagent is not required for siRNA transfection. In addition, this transfection reagent is not only suitable for transfection of a single plasmid or siRNA, but also for transfection of multiple plasmids or co-transfection of plasmids and siRNAs. Plasmid expression can be detectable 24-48 h after transfection, and siRNA silencing should be achieved 3-5 days after transfection.

When transfecting cells with this reagent, the presence of serum or antibiotics does not affect the transfection efficiency. However, for optimal transfection, it is recommended to use a serum-containing medium without antibiotics for transfection.

Figure 1: Lipo3K Transfection Reagent (Left) compared to Lipo2K Transfection Reagent (Right) transfected HEK293T cells