Contains the macro- and micronutrients, & vitamins as described by Linsmaier & Skoog (1964).

This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This is a subsequent optimization of the medium developed by Murashige and Skoog. Linsmaier's research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine-HCI was optimized from 0.1 to 0.4 mg/L. Linsmaier also noted that folic acid, ρ-aminobenzoic acid, L-glutamine,and ascorbic acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine-HCI and myo-Inositol.