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> Leukotriene B4 ELISA Kit

Leukotriene B4 ELISA Kit

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Cat# OKCD02276

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Leukotriene B4 ELISA Kit (OKCD02276)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)83-98%78-105%92-101%78-88%
EDTA plasma(n=5)93-101%78-88%80-97%91-104%
heparin plasma(n=5)92-99%78-90%91-101%91-104%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene B4 (LTB4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene B4 (LTB4) and unlabeled Leukotriene B4 (LTB4) (Standards or samples) with the pre-coated antibody specific to Leukotriene B4 (LTB4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene B4 (LTB4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene B4 (LTB4) in the sample.
ELISA Kit Range246.9-20,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-9592
EDTA plasma(n=5)94-10197
heparin plasma(n=5)86-9991
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukotriene B4 (LTB4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukotriene B4 (LTB4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Leukotriene B4 Microplate96 Wells (12 x 8 Well Strips)
Leukotriene B4 Lyophilized Standard2
100X Leukotriene B4 Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 71160-24-2
PubChem: 5280492
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sensitivity89.3 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolLTB4
Alias SymbolsLEUKOTRIENE B4; LTB4; 5,12-Dihete; 5,12-Hete
Protein NameLeukotriene B4
Description of TargetLEUKOTRIENE B4 is the major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)