Lep (Leptin) BioAssay™ ELISA Kit (Hamster)

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Cat# 519357-96T

Size : 96Tests

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

Contact local distributor :

Phone : +1 850 650 7790

Shipping Temp

Blue Ice

Storage Temp

4°C/-20°C

The Lep (Leptin) BioAssay™ ELISA Kit (Hamster) is a quantitative sandwich assay for the detection of Lep in serum, plasma, tissue homogenates and other biological fluids.

Sensitivity:

4.688pg/ml

Detection Range:

7.813-500pg/ml

Specificity:

This assay has high sensitivity and excellent specificity for detection of human Lep. No significant crossreactivity or interference betweenLepand analogues was observed.

Precision:

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Test Principle:

This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. Anti-Lep antibody was pre-coated onto 96-well plates. The biotin conjugated anti-Lep antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lep amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Lep can be calculated.

Kit Components:

Micro ELISA Plate(Dismountable), 8×12
Lyophilized Standard, 2x vial
Sample / Standard dilution buffer, 1x20ml
Biotin-labeled antibody (Concentrated), 1x120ul
Antibody dilution buffer, 1x10ml
HRP-Streptavidin Conjugate(SABC), 1x120ul
SABC dilution buffer, 1x10ml
TMB substrate, 1x10ml
Stop solution, 1x10ml
Wash buffer (25X), 1x30ml
Plate Sealer, 1x5pieces

Storage and Stability:

Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards