KLHL17 (NM_198317) Human Tagged ORF Clone

CAT#: RC212782

KLHL17 (Myc-DDK-tagged)-Human kelch-like 17 (Drosophila) (KLHL17)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

Datasheet
SDS
Plasmid Map

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KLHL17
Synonyms AF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212782 representing NM_198317
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCCCGCAGCGAGCGCCCGGCCGGCAGGACGCAGAGCCCGGAGCACGGCAGCCCGGGGCCCGGGC
CCGAGGCGCCGCCGCCTCCACCGCCGCAGCCGCCGGCCCCCGAGGCAGAGCGCACGCGGCCCCGGCAGGC
TCGGCCCGCAGCCCCCATGGAGGGAGCCGTGCAGCTGCTGAGCCGCGAGGGCCACAGCGTGGCCCACAAC
TCCAAGCGGCACTACCACGATGCCTTCGTGGCCATGAGCCGCATGCGCCAGCGCGGCCTCCTGTGCGACA
TCGTCCTGCACGTGGCTGCCAAGGAGATCCGTGCGCACAAAGTGGTGCTGGCCTCCTGCAGCCCCTACTT
CCACGCCATGTTCACAAATGAGATGAGCGAGAGCCGCCAGACCCACGTGACGCTGCACGACATCGACCCT
CAGGCCTTGGACCAGCTGGTGCAGTTTGCCTACACGGCTGAGATTGTGGTGGGCGAGGGCAATGTGCAGA
CTCTGCTCCCAGCCGCCAGTCTCCTGCAGCTGAATGGCGTCCGAGACGCTTGCTGCAAGTTTCTACTGAG
TCAGCTCGACCCCTCCAACTGCCTGGGTATCCGGGGCTTTGCCGATGCGCACTCCTGCAGCGACCTGCTC
AAGGCCGCCCACAGGTACGTGCTGCAGCACTTCGTGGACGTGGCCAAGACCGAGGAGTTTATGCTGCTGC
CCCTGAAACAGGTTCTGGAACTGGTCTCTAGCGACAGCCTGAACGTGCCTTCAGAGGAGGAGGTCTACCG
AGCCGTCCTGAGCTGGGTGAAACACGACGTGGACGCCCGCAGGCAGCATGTCCCACGGCTCATGAAGTGT
GTGCGGCTGCCCTTGCTGAGCCGCGACTTCCTGCTGGGCCACGTGGATGCCGAGAGCCTGGTGAGGCACC
ACCCTGACTGCAAGGACCTCCTCATCGAGGCCCTGAAGTTCCACCTGCTGCCTGAGCAGAGGGGCGTCCT
AGGCACCAGCCGCACACGTCCCCGGCGCTGCGAGGGGGCCGGGCCTGTGCTTTTTGCTGTGGGCGGCGGG
AGCCTGTTTGCCATCCACGGAGACTGTGAGGCCTACGACACGCGCACCGACCGCTGGCACGTGGTGGCCT
CCATGTCCACGCGCCGGGCCCGGGTGGGAGTGGCTGCGGTGGGGAACCGGCTCTATGCTGTGGGCGGCTA
TGATGGGACCTCAGACCTGGCTACCGTGGAGTCCTACGACCCCGTGACTAACACGTGGCAGCCGGAGGTG
TCCATGGGCACAAGGCGAAGCTGCCTGGGTGTGGCCGCCTTGCATGGACTCCTGTACTCGGCCGGCGGCT
ATGACGGGGCCTCCTGCCTGAACAGTGCTGAACGCTACGACCCCCTGACCGGAACGTGGACGTCCGTCGC
TGCCATGAGCACCCGGAGGCGCTATGTGCGAGTGGCCACGCTTGATGGGAACCTGTATGCTGTGGGCGGC
TACGACAGCTCCTCACACCTGGCCACTGTGGAGAAGTATGAGCCCCAGGTGAACGTGTGGTCGCCCGTGG
CGTCCATGCTGAGCCGACGCAGCTCAGCGGGCGTGGCCGTGCTGGAGGGTGCCCTGTACGTGGCAGGGGG
CAACGACGGCACCAGCTGCCTCAACTCGGTAGAGAGATACAGTCCAAAGGCTGGAGCCTGGGAAAGCGTG
GCGCCCATGAATATCCGCAGGAGCACGCATGACCTGGTGGCCATGGACGGATGGTTGTACGCCGTGGGGG
GTAACGACGGTAGCTCCAGCCTCAACTCCATCGAGAAGTACAACCCGAGGACCAACAAGTGGGTGGCCGC
ATCCTGCATGTTCACCCGGCGCAGCAGTGTGGGTGTGGCGGTGCTGGAGCTGCTCAATTTCCCGCCGCCA
TCCTCCCCGACGCTGTCCGTGTCCTCCACCAGCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC212782 representing NM_198317
Red=Cloning site Green=Tags(s)

MQPRSERPAGRTQSPEHGSPGPGPEAPPPPPPQPPAPEAERTRPRQARPAAPMEGAVQLLSREGHSVAHN
SKRHYHDAFVAMSRMRQRGLLCDIVLHVAAKEIRAHKVVLASCSPYFHAMFTNEMSESRQTHVTLHDIDP
QALDQLVQFAYTAEIVVGEGNVQTLLPAASLLQLNGVRDACCKFLLSQLDPSNCLGIRGFADAHSCSDLL
KAAHRYVLQHFVDVAKTEEFMLLPLKQVLELVSSDSLNVPSEEEVYRAVLSWVKHDVDARRQHVPRLMKC
VRLPLLSRDFLLGHVDAESLVRHHPDCKDLLIEALKFHLLPEQRGVLGTSRTRPRRCEGAGPVLFAVGGG
SLFAIHGDCEAYDTRTDRWHVVASMSTRRARVGVAAVGNRLYAVGGYDGTSDLATVESYDPVTNTWQPEV
SMGTRRSCLGVAALHGLLYSAGGYDGASCLNSAERYDPLTGTWTSVAAMSTRRRYVRVATLDGNLYAVGG
YDSSSHLATVEKYEPQVNVWSPVASMLSRRSSAGVAVLEGALYVAGGNDGTSCLNSVERYSPKAGAWESV
APMNIRRSTHDLVAMDGWLYAVGGNDGSSSLNSIEKYNPRTNKWVAASCMFTRRSSVGVAVLELLNFPPP
SSPTLSVSSTSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_198317
ORF Size 1926 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_198317.3
RefSeq Size 2560 bp
RefSeq ORF 1929 bp
Locus ID 339451
UniProt ID Q6TDP4
Cytogenetics 1p36.33
MW 69.7 kDa
Gene Summary The protein encoded by this gene is expressed in neurons of most regions of the brain. It contains an N-terminal BTB domain, which mediates dimerization of the protein, and a C-terminal Kelch domain, which mediates binding to F-actin. This protein may play a key role in the regulation of actin-based neuronal function. [provided by RefSeq, Aug 2010]

Other Versions

SKU Description Size
RC212782L1 Lenti ORF clone of Human kelch-like 17 (Drosophila) (KLHL17), Myc-DDK-tagged
    • 10 ug

898,00 EUR

RC212782L2 Lenti ORF clone of Human kelch-like 17 (Drosophila) (KLHL17), mGFP tagged
    • 10 ug

898,00 EUR

RC212782L3 Lenti ORF clone of Human kelch-like 17 (Drosophila) (KLHL17), Myc-DDK-tagged
    • 10 ug

898,00 EUR

RC212782L4 Lenti ORF clone of Human kelch-like 17 (Drosophila) (KLHL17), mGFP tagged
    • 10 ug

898,00 EUR

RG212782 KLHL17 (tGFP-tagged) - Human kelch-like 17 (Drosophila) (KLHL17)
    • 10 ug

798,00 EUR

SC314308 KLHL17 (untagged)-Human kelch-like 17 (Drosophila) (KLHL17)
    • 10 ug

658,00 EUR