KDM1A (NM_015013) Human Tagged ORF Clone
CAT#: RC208480
- TrueORF®
KDM1A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | KDM1A |
| Synonyms | AOF2; BHC110; CPRF; KDM1; LSD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RC208480 representing NM_015013 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTATCTGGGAAGAAGGCGGCAGCCGCGGCGGCGGCGGCTGCAGCGGCAGCAACCGGGACGGAGGCTG GCCCTGGGACAGCAGGCGGCTCCGAGAACGGGTCTGAGGTGGCCGCGCAGCCCGCGGGCCTGTCGGGCCC AGCCGAGGTCGGGCCGGGGGCGGTGGGGGAGCGCACACCCCGCAAGAAAGAGCCTCCGCGGGCCTCGCCC CCCGGGGGCCTGGCGGAACCGCCGGGGTCCGCAGGGCCTCAGGCCGGCCCTACTGTCGTGCCTGGGTCTG CGACCCCCATGGAAACTGGAATAGCAGAGACTCCGGAGGGGCGTCGGACCAGCCGGCGCAAGCGGGCGAA GGTAGAGTACAGAGAGATGGATGAAAGCTTGGCCAACCTCTCAGAAGATGAGTATTATTCAGAAGAAGAG AGAAATGCCAAAGCAGAGAAGGAAAAGAAGCTTCCCCCACCACCCCCTCAAGCCCCACCTGAGGAAGAAA ATGAAAGTGAGCCTGAAGAACCATCGGGTGTGGAGGGCGCAGCTTTCCAGAGCCGACTTCCTCATGACCG GATGACTTCTCAAGAAGCAGCCTGTTTTCCAGATATTATCAGTGGACCACAACAGACCCAGAAGGTTTTT CTTTTCATTAGAAACCGCACACTGCAGTTGTGGTTGGATAATCCAAAGATTCAGCTGACATTTGAGGCTA CTCTCCAACAATTAGAAGCACCTTATAACAGTGATACTGTGCTTGTCCACCGAGTTCACAGTTATTTAGA GCGTCATGGTCTTATCAACTTCGGCATCTATAAGAGGATAAAACCCCTACCAACTAAAAAGACAGGAAAG GTAATTATTATAGGCTCTGGGGTCTCAGGCTTGGCAGCAGCTCGACAGTTACAAAGTTTTGGAATGGATG TCACACTTTTGGAAGCCAGGGATCGTGTGGGTGGACGAGTTGCCACATTTCGCAAAGGAAACTATGTAGC TGATCTTGGAGCCATGGTGGTAACAGGTCTTGGAGGGAATCCTATGGCTGTGGTCAGCAAACAAGTAAAT ATGGAACTGGCCAAGATCAAGCAAAAATGCCCACTTTATGAAGCCAACGGACAAGCTGTTCCTAAAGAGA AAGATGAAATGGTAGAGCAAGAGTTTAACCGGTTGCTAGAAGCTACATCTTACCTTAGTCATCAACTAGA CTTCAATGTCCTCAATAATAAGCATGTGTCCCTTGGCCAGGCATTGGAAGTTGTCATTCAGTTACAAGAG AAGCATGTCAAAGATGAGCAGATTGAACATTGGAAGAAGATAGTGAAAACTCAGGAAGAATTGAAAGAAC TTCTTAATAAGATGGTAAATTTGAAAGAGAAAATTAAAGAACTCCATCAGCAATACAAAGAAGCATCTGA AGTAAAGCCACCCAGAGATATTACTGCCGAGTTCTTAGTGAAAAGCAAACACAGGGATCTGACCGCCCTA TGCAAGGAATATGATGAATTAGCTGAAACACAAGGAAAGCTAGAAGAAAAACTTCAGGAGTTGGAAGCGA ATCCCCCAAGTGATGTATATCTCTCATCAAGAGACAGACAAATACTTGATTGGCATTTTGCAAATCTTGA ATTTGCTAATGCCACACCTCTCTCAACTCTCTCCCTTAAGCACTGGGATCAGGATGATGACTTTGAGTTC ACTGGCAGCCACCTGACAGTAAGGAATGGCTACTCGTGTGTGCCTGTGGCTTTAGCAGAAGGCCTAGACA TTAAACTGAATACAGCAGTGCGACAGGTTCGCTACACGGCTTCAGGATGTGAAGTGATAGCTGTGAATAC CCGCTCCACGAGTCAAACCTTTATTTATAAATGCGACGCAGTTCTCTGTACCCTTCCCCTGGGTGTGCTG AAGCAGCAGCCACCAGCCGTTCAGTTTGTGCCACCTCTCCCTGAGTGGAAAACATCTGCAGTCCAAAGGA TGGGATTTGGCAACCTTAACAAGGTGGTGTTGTGTTTTGATCGGGTGTTCTGGGATCCAAGTGTCAATTT GTTCGGGCATGTTGGCAGTACGACTGCCAGCAGGGGTGAGCTCTTCCTCTTCTGGAACCTCTATAAAGCT CCAATACTGTTGGCACTAGTGGCAGGAGAAGCTGCTGGTATCATGGAAAACATAAGTGACGATGTGATTG TTGGCCGATGCCTGGCCATTCTCAAAGGGATTTTTGGTAGCAGTGCAGTACCTCAGCCCAAAGAAACTGT GGTGTCTCGTTGGCGTGCTGATCCCTGGGCTCGGGGCTCTTATTCCTATGTTGCTGCAGGATCATCTGGA AATGACTATGATTTAATGGCTCAGCCAATCACTCCTGGCCCCTCGATTCCAGGTGCCCCACAGCCGATTC CACGACTCTTCTTTGCGGGAGAACATACGATCCGTAACTACCCAGCCACAGTGCATGGTGCTCTGCTGAG TGGGCTGCGAGAAGCGGGAAGAATTGCAGACCAGTTTTTGGGGGCCATGTATACGCTGCCTCGCCAGGCC ACACCAGGTGTTCCTGCACAGCAGTCCCCAAGCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208480 representing NM_015013 Red=Cloning site Green=Tags(s) MLSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASP PGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEE RNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVF LFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGK VIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVN MELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKHVSLGQALEVVIQLQE KHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTAL CKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEF TGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVL KQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKA PILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSG NDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQA TPGVPAQQSPSM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_015013 |
| ORF Size | 2556 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015013.4 |
| RefSeq Size | 3030 bp |
| RefSeq ORF | 2559 bp |
| Locus ID | 23028 |
| UniProt ID | O60341 |
| Cytogenetics | 1p36.12 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 92.7 kDa |
| Gene Summary | This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
Documents
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| FAQs |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC208480L1 | Lenti ORF clone of Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2, Myc-DDK-tagged | 1 080,00 EUR | |
| RC208480L2 | Lenti ORF clone of Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2, mGFP tagged | 1 080,00 EUR | |
| RC208480L3 | Lenti ORF clone of Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2, Myc-DDK-tagged | 1 080,00 EUR | |
| RC208480L4 | Lenti ORF clone of Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2, mGFP tagged | 1 080,00 EUR | |
| RG208480 | KDM1A (tGFP-tagged) - Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2 | 980,00 EUR | |
| SC125274 | KDM1A (untagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2 | 781,00 EUR |