Il2ra rat monoclonal antibody, clone 3C7, Low Endotoxin
Specifications
Product Data
Clone Name
3C7
Application
FC, FN, IP
Recommended Dilution
Flow Cytometry: Identification and enumeration of CD25+ (Ref.1-4) Immunoprecipitation. (Ref.1,2,8,10) Functional Assay: Inhibition of IL-2 or mitogen-induced T cell proliferation. (Ref.13) In vitro blocking of IL-2 binding. (Ref.2,12)
Reactivity
Mouse
Antibody Host
Rat
Isotype
IgG2b
Clonality
Monoclonal
Specificity
This antibody is specific to Low affinity alpha chain IL-2 Receptor (IL-2Ra), Mr 55 kDa. The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. (Ref.2,11,12) Clone 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. (Ref.2,13)
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. (Ref.14) CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. (Ref.2,3,15) Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. (Ref.4) CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. (Ref.5) It is expressed at a higher level on CD4+CD8+ thymocytes. (Ref.5,6) It is also expressed on cultured epidermal Langerhans cells. (Ref.7) The biochemical features of murine CD25 have been characterized in detail. (Ref.8-10)
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