IL2 ELISA Kit (Horse)

Cat# OKCD00739

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for IL2 ELISA Kit (Horse) (OKCD00739)
Product Info
Predicted Species ReactivityEquus caballus|Horse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)79-90%78-94%90-98%83-99%
EDTA plasma(n=5)96-104%90-104%88-96%93-101%
heparin plasma(n=5)96-103%78-91%99-105%88-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 (IL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 (IL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 (IL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 2 (IL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9895
EDTA plasma(n=5)80-10399
heparin plasma(n=5)84-9791
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IL2 Microplate96 Wells (12 x 8 Well Strips)
IL2 Lyophilized Standard2 x 2 ng
100X Biotinylated IL2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity< 10.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 2 (IL2). No significant cross-reactivity or interference between Interleukin 2 (IL2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL2
Gene Full Nameinterleukin 2
Alias SymbolsIL-2;interleukin-2;interleukin-2 precursor protein;T-cell growth factor;TCGF.
NCBI Gene Id100034204
Protein NameInterleukin-2
Description of TargetProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Uniprot IDP37997
Protein Accession #NP_001078902
Nucleotide Accession #NM_001085433.2
Protein Size (# AA)149