IL-8 ELISA Kit (Human)
Cat# OKBB00192
Size : 96wells/kit,withremovablestrips.
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Human | |||||||||||||||||||||||||||||||||||
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| Application | ELISA | |||||||||||||||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | |||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | |||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva's human IL-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-8 has been precoated onto 96-well plates. Standards(E.coli, S28-S99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-8 amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 15.6 pg/ml - 1,000 pg/ml | |||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Additional Information | Range: 15.6pg/ml-1000pg/ml (cell culture supernates) 7.8pg/ml-500pg/ml ( serum, plasma). | |||||||||||||||||||||||||||||||||||
| :: | Principle: Aviva’s human IL-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-8 has been precoated onto 96-well plates. Standards(E.coli, S28-S99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-8 amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
| :: | Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended. 2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case. 3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes. 4. Duplicate well assay is recommended for both standard and sample testing. 5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate. 6. Don’t reuse tips and tubes to avoid cross contamination. 7. To avoid to use the reagents from different batches together. 8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using. Background: Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils.1 IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.2 The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside.1 Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form. | |||||||||||||||||||||||||||||||||||
| Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | |||||||||||||||||||||||||||||||||||
| Sample Type | cell culture supernates, serum and plasma (heparin, EDTA, citrate) | |||||||||||||||||||||||||||||||||||
| Sensitivity | <1 pg/ml | |||||||||||||||||||||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | No detectable cross-reactivity with any other cytokine. | |||||||||||||||||||||||||||||||||||
| Reference | 1. Modi, W. S.; Dean, M.; Seuanez, H. N.; Mukaida, N.; Matsushima, K.; O'Brien, S. J. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. Hum. Genet. 84: 185-187, 1990. 2. Bruun, J. M.; Pedersen, S. B.; Richelsen, B. Regulation of interleukin 8 production and gene expression in human adipose tissue in vitro. J. Clin. Endocr. Metab. 86: 1267-1273, 2001. | |||||||||||||||||||||||||||||||||||
| Specificity | Natural and recombinant human IL-8 | |||||||||||||||||||||||||||||||||||
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: S28-S99 | |||||||||||||||||||||||||||||||||||
| Description | For quantitative detection of human IL-8 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate). | |||||||||||||||||||||||||||||||||||
| Gene Symbol | CXCL8 |
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| Gene Full Name | chemokine (C-X-C motif) ligand 8 |
| Alias Symbols | (Ala-IL-8)77, (Ser-IL-8)72, 3 10C, 3-10C, AMCF I, AMCF-I, AMCFI, b ENAP, B-ENAP, Beta-thromboglobulin-like protein, C X C motif chemokine 8, C-X-C motif chemokine 8, Chemokine (C X C motif) ligand 8, CXCL8, Emoctakin, GCP 1, GCP-1, GCP/IL-8 protein I, GCP/IL-8 protein II, GCP/IL-8 protein III, GCP/IL-8 protein IV, GCP/IL-8 protein V, GCP/IL-8 protein VI, GCP1, Granulocyte chemotactic protein 1, IL 8, IL-8, IL-8(1-77), IL-8(9-77), IL8, IL8/NAP1 form I, IL8/NAP1 form II, IL8/NAP1 form III, IL8/NAP1 form IV, IL8/NAP1 form V, IL8/NAP1 form VI, IL8_HUMAN, Interleukin 8, K60, LECT, LUCT, Lymphocyte derived neutrophil activating factor, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF, MDNCF-b, MDNCF-c, MONAP, Monocyte derived neutrophil activating peptide, Monocyte derived neutrophil chemotactic factor, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAF, NAP 1, NAP-1, NAP1, Neutrophil activating peptide 1, Neutrophil activating protein 1, Neutrophil-activating factor, Neutrophil-activating protein 1, Protein 3 10C, Protein 3-10C, SCYB8, Small inducible cytokine subfamily B member 8, T cell chemotactic factor, T-cell chemotactic factor, TSG 1, TSG-1, TSG1 |
| NCBI Gene Id | 3576 |
| Protein Name | Interleukin-8 |
| Description of Target | Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils.1 IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.2 The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside.1 Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form. |
| Uniprot ID | P10145 |
| Protein Accession # | NP_000575.1 |
| Nucleotide Accession # | NM_000584.3 |