IL-1beta (Human) Colorimetric Sandwich ELISA Kit
Cat# OK-0227
Size : 1Kit
Brand : Assay Biotechnology
Contact local distributor :
Phone : +1 850 650 7790
Human 4-1BB-R recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human 4-1BB-R was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
A short protocol of the steps taken in Assay Biotechnology's OmniKine Sandwich ELISA Kits.
An illustrated breakdown of the traditional colorimetric sandwich assay principles.
The data and subsequent graph were obtained after performing a cytokine ELISA for 4-1BB-R. Each known sample concentration was assayed in triplicate. The OmniKine™ Human 4-1BB-R ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human 4-1BB-R proteins within the range of 16-2000 pg/ml.