OPPA00534 | IL 18 Protein Biotrend

Cat# OPPA00534

Size : 5ug

Contact local distributor :

Phone : +1 850 650 7790

Brand : Aviva Systems Biology

Contact local distributor :

Phone : +1 850 650 7790

Datasheets/Manuals	Printable datasheet for OPPA00534

Product Info
Predicted Species Reactivity	Human
Product Format	Lyophilized 0.5x PBS (pH 7.3)
Host	E. Coli
Reconstitution and Storage	It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18M-omega-cm H₂O not less than 100 ug/mL, which can then be further diluted to other aqueous solutions. Lyophilized Interleukin 18 although stable at room temperature for 3 weeks, should be stored dessicated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA and BSA). Please prevent freeze/thaw cycles.
Purification	Purified by proprietary chromatographic techniques.
Concentration	1 mg/ml (prior to lyophilization)
Purity	Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity	The ED₅₀ as determined by the dose-dependant stimulation of the IFN-g by human PBMC co-stimulated with IL-12 was found to be 5 ng/ml corresponding to a Specific Activity of 200,000 IU/mg.

Gene Symbol	IL18
Alias Symbols	IGIF, IL-18, IL-1g, IL1F4
NCBI Gene Id	3606
Protein Name	Interleukin-18
Description of Target	IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. Interleukin-18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids.
Uniprot ID	Q14116
Protein Accession #	NP_001553.1
Protein Size (# AA)	Recombinant
Molecular Weight	18 kDa

Protein interactions

Name	# of Products
CASP1	98
CASP3	324
CASP4	79
IL18BP	5
IL18R1	15
IL18RAP	14
IL1RL2	5

Biological pathways

Name	# of Products
Angiogenesis	366
Cell-cell signaling	763
Chemokine biosynthetic process	5
Granulocyte macrophage colony-stimulating factor biosynthetic process	5
Interferon-gamma biosynthetic process	23
Interleukin-13 biosynthetic process	6
Interleukin-2 biosynthetic process	5
Positive regulation of activated T cell proliferation	123
Positive regulation of granulocyte macrophage colony-stimulating factor production	23
Positive regulation of inflammatory response	172
Positive regulation of interferon-gamma production	108
Positive regulation of interleukin-17 production	72
Positive regulation of natural killer cell proliferation	23
Positive regulation of NK T cell proliferation	16
Positive regulation of tissue remodeling	50
Regulation of cell adhesion	64
Sleep	11
T-helper 1 type immune response	37
Type 2 immune response	21

Biological process

Name	# of Products
Angiogenesis	196
Cell-cell signaling	235
Cellular response to cAMP	28
Cellular response to hydrogen peroxide	38
Cellular response to interferon-gamma	26
Cellular response to mechanical stimulus	62
Cellular response to organic cyclic compound	58
Cellular response to parathyroid hormone stimulus	6
Cellular response to peptide hormone stimulus	16
Cellular response to tumor necrosis factor	39
Chemokine biosynthetic process	1
Detection of mechanical stimulus involved in sensory perception of pain	7
Digestive tract morphogenesis	23
Granulocyte macrophage colony-stimulating factor biosynthetic process	1
Immune response	404
Inflammatory response	339
Interferon-gamma biosynthetic process	8
Interleukin-13 biosynthetic process	2
Interleukin-2 biosynthetic process	2
Lipopolysaccharide-mediated signaling pathway	48
Lung development	105
MAPK cascade	105
Osteoblast differentiation	57
Positive regulation of activated T cell proliferation	45
Positive regulation of chemokine production	34
Positive regulation of collagen biosynthetic process	29
Positive regulation of gene expression	160
Positive regulation of granulocyte macrophage colony-stimulating factor production	13
Positive regulation of inflammatory response	57
Positive regulation of interferon-gamma production	61
Positive regulation of interleukin-1 beta production	6
Positive regulation of interleukin-17 production	27
Positive regulation of interleukin-8 production	33
Positive regulation of male germ cell proliferation	3
Positive regulation of natural killer cell proliferation	14
Positive regulation of neuron apoptosis	53
Positive regulation of NK T cell proliferation	8
Positive regulation of superoxide anion generation	6
Positive regulation of tissue remodeling	6
Positive regulation of tumor necrosis factor production	64
Positive regulation of vascular permeability	16
Regulation of blood vessel remodeling	3
Regulation of cell adhesion	41
Response to cold	31
Response to hyperoxia	20
Response to hypoxia	205
Response to radiation	32
Sleep	21
T-helper 1 type immune response	14
Type 2 immune response	3

Cellular components

Name	# of Products
Apical plasma membrane	173
Cytoplasm	4549
Cytosol	1868
Extracellular region	1573
Extracellular space	884

Protein function

Name	# of Products
Cytokine activity	1104

Titration: 1.0 ug/ml
Positive Control: Hela Whole CellIL18 is supported by BioGPS gene expression data to be expressed in HeLa" data-size="300x300" class="product-image-wrapper cover">

IL18 Antibody - C-terminal region (ARP60740_P050)
Catalog #: ARP60740_P050

Species Tested: Human

Application: WB

Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
All lanes: Anti IL18 (OABB01190) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 22 kDa
Observed bind size: 22 kDa" data-size="300x300" class="product-image-wrapper cover">

IL18 Antibody (OABB01190)
Catalog #: OABB01190

Clone: Polyclonal

Species Tested: Human

Application: Immunohistochemistry|Western blot

Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Size: 100UG
IL18 ELISA Kit (Human) : 96 Wells (OKEH00105)
Catalog #: OKEH00105

Application: Enzyme-linked Immunosorbent assay-Sandwich

Kit Range: 15.6-1000pg/mL

Sensitivity: 10 pg/mL

Size: 96T
IL18 Antibody (OAAN00220)
Catalog #: OAAN00220

Conjugation: Unconjugated

Application: WB, IHC

Format: Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
IL18 ELISA Kit (Mouse) (OKEH04042)
Catalog #: OKEH04042

Application: Enzyme-linked Immunosorbent assay-Sandwich

Kit Range: 15.6-1000pg/mL

Sensitivity: 7.87 pg/mL

Size: 96T

IL 18 Protein

Cat# OPPA00534

Contact local distributor :

Brand : Aviva Systems Biology

Contact local distributor :

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