Igf2 ELISA Kit (Hamster)
Cat# OKBB00712
Size : 96wells/kit,withremovablestrips.
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
|---|
| Predicted Species Reactivity | Hamster | ||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva's Hamster IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-2 has been precoated onto 96-well plates. Standards(E.coli, A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Hamster IGF-2 amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 62.5 pg/ml - 4,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
| ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||||||||||||||||||||||
| Additional Information | To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
*number of samples for each test n=16. | ||||||||||||||||||||||||||||||||||||||||||
| :: |
| ||||||||||||||||||||||||||||||||||||||||||
| Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster IGF-2 | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | <5 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A25-E91 | ||||||||||||||||||||||||||||||||||||||||||
| Description | Sandwich high sensitivity ELISA kit for Quantitative Detection of Chinese hamster IGF-2. 96wells/kit, with removable strips. | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | IGF2 |
|---|---|
| Gene Full Name | Insulin-like growth factor II |
| Alias Symbols | Insulin-like growth factor II;IGF-II;Multiplication-stimulating activity;MSA;Multiplication-stimulating polypeptide;Insulin-like growth factor II;Preptin;Igf2;Igf-2; |
| NCBI Gene Id | 100762359 |
| Protein Name | Insulin-like growth factor II |
| Description of Target | Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. |
| Uniprot ID | P01346 |
| Protein Accession # | NP_001177091.1 |
| Nucleotide Accession # | NM_001190162.1 |