A2515-200mg | Hygromycin B [31282-04-9] Biotrend

Hygromycin B is a selective antibiotic.

Antibiotics are a type of antimicrobial used in the treatment and prevention of bacterial infection.

Hygromycin B is an antibiotic that is effective on most bacteria, fungi and higher eukaryotes. Hygromycin B has a single binding site within the 30S subunit, which is in consistent with its monophasic effect. The binding site is located in the major groove of the helix with a highly sequence-specific way. Hygromycin B inhibits protein synthesis by inhibiting the translocation step of elongation and causing mistranslation of mRNA in the 70S ribosome [1]. Hygromycin B selectively inhibited activity of the ribosomal ATPase (RbbA) on 70S ribosomes and releases RbbA from 70S ribosomes at physiological concentrations. RbbA enhances the reactivity of A889 and G890 of the 16S rRNA switch helix region. Also, Hygromycin B protects G1494 and A1408 in the decoding region.

References:
[1] Brodersen DE, Clemons WM Jr, Carter AP, et al. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell, 2000, 103 (7): 1143-1154.
[2] Ganoza MC, Kiel MC. A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob Agents Chemother, 2001, 45 (10): 2813-2819.

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	527.5
Cas No.	31282-04-9
Formula	C₂₀H₃₇N₃O₁₃
Solubility	≥26.38 mg/mL in H2O; ≥5.35 mg/mL in EtOH; ≥9.14 mg/mL in DMSO
Chemical Name	(3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
SDF	Download SDF
Canonical SMILES	CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Cell experiment:[1]
Cell lines	Mouse L cells
Reaction Conditions	200 μg/ml hygromycin B for 2 ~ 6 weeks
Applications	Hygromycin B was used for resistance selection in mouse L cells transfected withEscherichia coli genes coding for resistance to the aminocyclitol antibiotics hygromycin B.
Animal experiment:[2]
Animal models	Four-week-old BALB/c mice susceptible to MHV-A59 infection
Dosage form	0 ~ 10 μmol/kg Intraperitoneal (i.p.) injection
Applications	A single injection of hygromycin B reduced the number of lesions in the livers of MHV-A59-inoculated mice. Higher doses of the drug appeared to be slightly more effective at reducing virus-induced lesions.
Note	The technical data provided above is for reference only.
	References: 1. Santerre RF, Allen NE, Hobbs JN Jr, et al. Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells. Gene, 1984, 30(1-3): 147-156. 2. Macintyre G, Curry B, Wong F, et al. Hygromycin B therapy of a murine coronaviral hepatitis. Antimicrobial Agents and Chemotherapy, 1991, 35(10): 2125-2127.

Purity = 98.00%

Chemical structure

Hygromycin B [31282-04-9]

Cat# A2515-200mg

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Brand : ApexBio Technology

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Chemical Properties

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Quality Control

Chemical structure

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