Specifications

Product Data
Format	15 x 96-well
Assay Type	Solid Phase Sandwich ELISA
Assay Length	From sample to end, 2 h to 4 h
Signal	Colorimetric
Curve Range	25-800 pg/ml
Sample Type	Serum, Plasma, Cell culture supernatant
Sample Volume	100 µl
Specificity	Recognizes both natural and recombinant human TNF-a
Sensitivity	10 pg/ml
Cross Reactivity	No cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.
Components	ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
Background	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Gene Symbol	TNF
Gene ID	7124