Human PARP2 antibody
Cat# ATGA0578-100
Size : 100ul
Brand : NKMAX BIO
ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0578 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: SW480 cell lysate
Lane 1.: SW480 cell lysate
The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 1.: HeLa cell lysate
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: NIH3T3 cell lysate
Lane 1.: Raji cell lysate
Lane 1.: NIH3T3 cell lysate
Lane 1.: Raji cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0578 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.