Human LTF (Lactotransferrin) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	LTF
Gene ID:	4057
Uniprot ID:	TRFL_HUMAN
Specificity:	This kit recognizes Human LTF/LF in samples. No significant cross-reactivity or interference between Human LTF/LF and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Isoform DeltaLf: Phosphorylation at Ser-10 activates the transcriptional activity. Phosphorylation at Ser-10 also promotes proteasomal degradation. Alternatively can undergo O-GlcNAcylation at Ser-10. Isoform DeltaLf: O-GlcNAcylation at Ser-10 inhibits DNA binding and negatively regulates the transcriptional activity. Alternatively can undergo phosphorylation at Ser-10. Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation.
Function	Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin: Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity, which depends on the extracellular cation concentration. Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Can also prevent bacterial biofilm development in P.aeruginosa infection. Has weak antifungal activity against C.albicans. Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection. Can inhibit papillomavirus infections. Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling. Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells. Stimulates VEGFA-mediated endothelial cell migration and proliferation. Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs). Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA. Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection. N-terminal region shows strong antifungal activity against C.albicans. Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. Kaliocin-1: Has antimicrobial activity and is able to permeabilize different ions through liposomal membranes. Lactoferroxin-A: Has opioid antagonist activity. Shows preference for mu-receptor. Lactoferroxin-B: Has opioid antagonist activity. Shows higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferroxin-C: Has opioid antagonist activity. Shows higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites. Isoform DeltaLf: Transcription factor with antiproliferative properties and ability to induce cell cycle arrest. Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELENOH promoters.
Protein Name	Lactotransferrin Lactoferrin Growth-Inhibiting Protein 12 Talalactoferrin Cleaved Into - Lactoferricin-H Lfcin-H - Kaliocin-1 - Lactoferroxin-A - Lactoferroxin-B - Lactoferroxin-C
Database Links	Reactome: R-HSA-1222449 Reactome: R-HSA-6798695 Reactome: R-HSA-6799990 Reactome: R-HSA-6803157 Reactome: R-HSA-977225
Cellular Localisation	Isoform 1: Secreted Cytoplasmic Granule Secreted Into Most Exocrine Fluids By Various Endothelial Cells Stored In The Secondary Granules Of Neutrophils Isoform Deltalf: Cytoplasm Nucleus Mainly Localized In The Cytoplasm
Alternative ELISA Names	Lactotransferrin ELISA kit Lactoferrin ELISA kit Growth-Inhibiting Protein 12 ELISA kit Talalactoferrin Cleaved Into - Lactoferricin-H ELISA kit Lfcin-H - Kaliocin-1 - Lactoferroxin-A - Lactoferroxin-B - Lactoferroxin-C ELISA kit LTF ELISA kit GIG12 ELISA kit LF ELISA kit
Specificity	This kit recognizes Human LTF/LF in samples. No significant cross-reactivity or interference between Human LTF/LF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-98	91
EDTA plasma (n=8)	89-103	95
Cell culture media (n=8)	94-107	100

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.00	2.10	9.10	1.00	2.20	9.30
Standard deviation	0.10	0.10	0.40	0.10	0.10	0.40
CV (%)	10.00	4.76	4.40	10.00	4.55	4.30

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