Human L2000 Array, Glass Slide

Cat# AAH-BLG-2000-8

Size : 8SampleKit

Brand : RayBiotech

Contact local distributor :


Phone : +1 850 650 7790
Human L2000 Array, Glass Slide

Human L2000 Array, Glass Slide

RayBio® L-Series Human Antibody Array 2000 Glass Slide Kit. Detects 2000 Human Proteins. Suitable for serum, plasma and cell culture supernatants. (4 or 8 Sample Kit)

Only %1 left
+

Product Description

Specifications

Size 4 Sample Kit, 8 Sample Kit
Species Human
Quantitative/Semi-Quantitative Semi-Quantitative
Number of Targets Detected 2000
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
Solid Support Glass Slide
Method Of Detection Fluorescence Laser Scanner
Design Principle Label-based

Product Features

  • High density arrays
  • Unbiased detection through direct biotin labeling of samples
  • Low sample consumption
  • High detection sensitivity
  • Same day result
  • Affordable and simple to use

Target Names

11b-HSD1, 14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 2B4, 2Mannosidase IA, 4-1BB, 53BP1, 6Ckine, A1BG, A2M, ABAT, ABCF1, ABI3BP, ABL1, ACAA, ACAA1, ACAA2, ACACA, ACE, ACE-2...View all 2000 human target proteins

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Antibody Array Glass Slide(s)
  • Spin Columns
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Labeling Buffer
  • Lysis Buffer
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline
  1. Purify Samples with Spin Column
  2. Biotinylate Samples
  3. Purify biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.