Specifications

Product Data
Format	10 x 96-well
Assay Type	Solid Phase Sandwich ELISA
Assay Length	From sample to end 2h45min
Signal	Colorimetric
Curve Range	12.5-400 pg/ml
Sample Type	Serum, Plasma, Cell culture supernatant
Sample Volume	100 µl
Specificity	Recognizes both natural and recombinant human IL-10
Sensitivity	5 pg/ml
Cross Reactivity	No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10. No cross reactivity with viral IL-10.
Components	ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
Background	The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2011]
Gene Symbol	IL10
Gene ID	3586