General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This human HYOU1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | HYOU1 |
| Gene ID: | 10525 |
| Uniprot ID: | HYOU1_HUMAN |
| Specificity: | This kit recognizes Human HYOU1 in samples. No significant cross-reactivity or interference between Human HYOU1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Tissue Specificity | Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. |
| Function | Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. |
| Protein Name | Hypoxia Up-Regulated Protein 1150 Kda Oxygen-Regulated ProteinOrp-150170 Kda Glucose-Regulated ProteinGrp-170 |
| Database Links | Reactome: R-HSA-3000484Reactome: R-HSA-381038 |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative CLIA Names | Hypoxia Up-Regulated Protein 1 CLIA kit150 Kda Oxygen-Regulated Protein CLIA kitOrp-150 CLIA kit170 Kda Glucose-Regulated Protein CLIA kitGrp-170 CLIA kitHYOU1 CLIA kitGRP170 CLIA kitORP150 CLIA kit |
| Specificity | This kit recognizes Human HYOU1 in samples. No significant cross-reactivity or interference between Human HYOU1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 96 |
| EDTA plasma(n=8) | 98-112 | 104 |
| Cell culture media(n=8) | 88-100 | 93 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 92.55 | 208.06 | 994.36 | 91.87 | 214.60 | 923.19 |
| Standard deviation | 11.22 | 17.81 | 111.67 | 9.52 | 24.68 | 92.78 |
| CV (%) | 12.12 | 8.56 | 11.23 | 10.36 | 11.50 | 10.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance