General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This DEFA5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | DEFA5 |
| Gene ID: | 1670 |
| Uniprot ID: | DEF5_HUMAN |
| Specificity: | This kit recognizes Human DEF Alpha 5 in samples. No significant cross-reactivity or interference between Human DEF Alpha 5 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Post Translational Modifications | Glycosylated. Proteolytically cleaved at Arg-62 by trypsin. Both the propeptide form proHD5/HD5(20-94) and HD5(56-94) are cleaved into the lumenal peptide form HD5(63-94) by trypsin. Unprocessed proHD5 exerts antimicrobial activities, but peptide potency is enhanced by peptide processing. Proteolytically cleaved in duodenal fluid.derived fragments are antimicrobially active against commensal bacteria (in vitro). (Microbial infection) The disulfide bridges and homodimerization are a prerequisite for the enhancement of S.flexneri adhesion and invasion. |
| Function | Host-defense peptide that maintains sterility in the urogenital system. Has antimicrobial activity against a wide range of bacteria, including Gram-negative E.coli, P.aeruginosa and S.typhimurium, and Gram-positive E.aerogenes, S.aureus, B.cereus, E.faecium and L.monocytogenes. Confers resistance to intestinal infection by S.typhimurium. Exhibits antimicrobial activity against enteric commensal bacteria such as B.adolescentis, L.acidophilus, B.breve, L.fermentum, B.longum and S.thermophilus. Binds to bacterial membranes and causes membrane disintegration. Induces the secretion of the chemokine IL-8 by intestinal epithelial cells. Binds to B.antracis lef/lethal factor, a major virulence factor from B.anthracis, and neutralizes its enzymatic activity. (Microbial infection) Acts as a target for S.flexneri infection by binding to the bacterium, possibly via bacterial surface proteins, and thereby augmenting infectivity via enhanced bacterial adhesion and invasion of epithelial cells and tissues. |
| Protein Name | Defensin Alpha 5Defensin-5Hd5(20-94 Cleaved Into - Hd5(23-94 - Hd5(29-94 - Hd5(56-94 - Hd5(63-94 |
| Database Links | Reactome: R-HSA-1461973Reactome: R-HSA-1462054 |
| Cellular Localisation | SecretedCytoplasmic VesicleSecretory VesicleStored As Propeptide Hd5(20-94) In Secretory Granules Of Small Intestinal Paneth Cells And Found In The Ileum Lumen As Processed Mature PeptidesPredominantly In The Hd5(63-94) FormPeptides Hd5(20-94)Hd5(23-94) And Hd5(29-94) Are Found Within TissuesHd5(20-94) Being The Predominant Intracellular FormPeptides Hd5(56-94) And Hd5(63-94) Are Found In The Extracellular MilieuHd5(63-94) Being The Most Abundant FormSecreted Into The Female Genital Tract Lumen |
| Alternative ELISA Names | Defensin Alpha 5 ELISA kitDefensin-5 ELISA kitHd5(20-94 Cleaved Into - Hd5(23-94 - Hd5(29-94 - Hd5(56-94 - Hd5(63-94 ELISA kitDEFA5 ELISA kitDEF5 ELISA kit |
| Specificity | This kit recognizes Human DEFα5 in samples. No significant cross-reactivity or interference between Human DEFα5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-105 | 99 |
| EDTA plasma (n=8) | 90-104 | 97 |
| Cell culture media (n=8) | 87-99 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.38 | 92.81 | 486.57 | 45.03 | 99.71 | 477.42 |
| Standard deviation | 2.72 | 5.10 | 25.25 | 2.48 | 5.71 | 24.92 |
| CV (%) | 5.62 | 5.50 | 5.19 | 5.51 | 5.73 | 5.22 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance