General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38mIU/mL |
| Detection Limit: | 15.63~1000mIU/mL |
| Short Description: | This CGB3; CGB5; CGB8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | CGB3.CGB5.CGB8 |
| Gene ID: | 1082/93659/94115 |
| Uniprot ID: | CGB3_HUMAN |
| Specificity: | This kit recognizes Human CG Beta in samples. No significant cross-reactivity or interference between Human CG Beta and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. |
| Protein Name | Choriogonadotropin Subunit Beta 3Choriogonadotropin Subunit BetaCg-BetaChorionic Gonadotropin Chain Beta |
| Database Links | Reactome: R-HSA-209822Reactome: R-HSA-8866910 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Choriogonadotropin Subunit Beta 3 ELISA kitChoriogonadotropin Subunit Beta ELISA kitCg-Beta ELISA kitChorionic Gonadotropin Chain Beta ELISA kitCGB3 ELISA kitCGB.CGB5.CGB8 ELISA kit |
| Specificity | This kit recognizes Human CGβ in samples. No significant cross-reactivity or interference between Human CGβ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-100 | 94 |
| EDTA plasma (n=8) | 90-100 | 95 |
| Cell culture media (n=8) | 93-105 | 100 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (mIU/mL) | 40.39 | 109.68 | 450.13 | 43.13 | 113.96 | 439.91 |
| Standard deviation | 2.06 | 5.47 | 18.19 | 2.32 | 5.64 | 22.88 |
| CV (%) | 5.10 | 4.99 | 4.04 | 5.38 | 4.95 | 5.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance