Human anti-2019 nCoV (N) IgG ELISA Kit
Cat# EH4395
Size : 96T
Brand : FineTest
Human anti-2019-nCoV(N) IgG ELISA Kit
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-2019 nCoV(N) IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-2019 nCoV(N) IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.