Human ACE/Cd143 ELISA Kit PicoKine®

Cat# EK0557

Size : 96wells/kit,withremovablestrips.

Brand : Boster Immunoleader

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Product Info Summary

SKU: EK0557
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) and saliva.

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Product info

Product Name

Human ACE/Cd143 ELISA Kit PicoKine®

View all ACE/CD143 ELISA kits

SKU/Catalog Number

EK0557

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ACE/Cd143 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ACE/Cd143 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0557)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-L1261

Sensitivity

<5 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0557 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0557 is reactive to ACE in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) and saliva.

Application Guarantee

EK0557 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ACE/CD143

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

Kit Components

Catalog Number Description Quantity
EK0557-CAP Anti-Human ACE Pre-coated 96-well strip microplate 1
EK0557-ST Human ACE Standard 2 vials, 50 ng/tube
EK0557-DA Human ACE Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Data Examples, Quality Control Data & Sample Dilution

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0390.0990.1830.2990.5120.9161.3711.923

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect ACE/CD143, Dilution ratio of 1:20, concentration in serum and plasma is 29-253 ng/ml..

Some articles we found to cite concentrations of ACE/CD143 in samples: 20541774, PMC9728626 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ACE/Cd143 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12786839241571358635025528
Standard deviation62.62465.051087.0686.57482.61225.34
CV (%)4.9%6.8%4.5%6.5%7.6%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ACE/CD143 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11278131012921425132658.134.3%
Sample 268396994703966696885145.232.1%
Sample 324157248842220721600232121350.665.8%
*number of samples for each test n=16.

Protein Target Info & Infographic

Gene/Protein Information For ACE (Source: Uniprot.Org, NCBI)

Gene Name

ACE

Full Name

Angiotensin-converting enzyme

Weight

149.715kDa

Superfamily

peptidase M2 family

Alternative Names

ACE; ACE1angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; CD143 antigen; CD143; DCP; DCP1; DCP1angiotensin-converting enzyme; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; EC 3.2.1.-; EC 3.4.15.1; Kininase II; MGC26566; MVCD3; peptidase P; testicular ECA ACE ACE1, CD143, DCP, DCP1 angiotensin I converting enzyme angiotensin-converting enzyme|CD143 antigen|angiotensin I converting enzyme (peptidyl-dipeptidase A) 1|carboxycathepsin|dipeptidyl carboxypeptidase 1|dipeptidyl carboxypeptidase I|kininase II|peptidase P

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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