HSP90AA1 ELISA Kit (Bovine)
Cat# OKEH06998
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Hsp90aa1 ELISA Kit (Bovine) (OKEH06998) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsp90aa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hsp90aa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsp90aa1 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 0.284 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Heat shock protein HSP 90-alpha | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | HSP90AA1 |
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| Gene Full Name | heat shock protein 90 alpha family class A member 1 |
| Alias Symbols | heat shock 90kD protein 1, alpha;heat shock protein 90kDa alpha (cytosolic), class A member 1;heat shock protein HSP 90-alpha;HSPCA. |
| NCBI Gene Id | 281832 |
| Protein Name | Q76LV2 |
| Description of Target | Heat shock protein HSP 90-alpha |
| Uniprot ID | https://www.uniprot.org/uniprot/Q76LV2 |
| Protein Accession # | NP_001012688 |
| Nucleotide Accession # | NM_001012670 |