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> HSP90 alpha/beta Antibody

HSP90 alpha/beta Antibody

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Cat# ASM10049-A390-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - HSP90 alpha/beta Antibody ASM10049
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (ASM10049). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (ASM10049) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.
  • IHC - HSP90 alpha/beta Antibody ASM10049
    Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A (ASM10049). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (ASM10049) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
  • WB - HSP90 alpha/beta Antibody ASM10049
    Western Blot analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A (ASM10049). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (ASM10049) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • ICC/IF - HSP90 alpha/beta Antibody ASM10049
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (ASM10049). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (ASM10049) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P08238, P07900
Other Accession NP_031381.2, NP_001017963.2
Host Mouse
Isotype IgG2a
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human HSP90 alpha/beta Monoclonal IgG2a
Target/Specificity Detects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.
Other Names HSP86 Antibody, HSP89A Antibody, HSP90AA1 Antibody, HSP90Alpha Antibody, HSPC1 Antibody, HSPCA Antibody, HSPCAL3 Antibody, HSP84 Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody, D6S182 Antibody, FLJ26985 Antibody
Clone Names Hyb-K41220A
Immunogen Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Cellular Localization Cytoplasm | Melanosome