ASM10354-A700-100 | HSC70 (HSP73) Antibody Biotrend

Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.
Western blot analysis of Human Cell line lysates showing detection of HSC70 protein using Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (ASM10354) at 1:50000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, IP, ICC
Primary Accession	P11142
Other Accession	NP_006588.1
Host	Rabbit
Reactivity	Human, Mouse, Rat, Hamster
Clonality	Polyclonal
Description	Rabbit Anti-Human HSC70 (HSP73) Polyclonal
Target/Specificity	Detects 73kDa. Does not cross-react with HSP70.
Other Names	HSC54 Antibody, HSC71 Antibody, HSC73 Antibody, HSP71 Antibody, HSP73 Antibody, HSPA10 Antibody, HSPA8 Antibody, LAP1 Antibody, NIP71 Antibody
Immunogen	Amino acids 650-670 of human HSP73
Purification	Rabbit Antiserum
Storage	4ºC
Storage Buffer	Rabbit Antiserum
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	0.5 µg/ml of SPC-102 was sufficient for detection of HSC70 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm \| Melanosome

HSC70 (HSP73) Antibody