K1070-5ml | HotStart™ 2X Green qPCR Master Mix Biotrend

Product Citation

1. Daohong He, Huasheng Wu, et al. "Se/GSH-enriched Candida utilis ameliorates DSS-induced colitis in mice by preventing oxidative stress and improving immune responses." Food Bioscience Volume 58, April 2024, 103656.
2. Runjie Song, Peilan Guo, et al. "A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer." Mol Cancer. 2023 Jul 5;22(1):104. PMID: 37408008
3. Xiaotian He, Dechang Zhao, et al. "Intrinsic Immunogenic Tumor Cell Death Subtypes Delineate Prognosis and Responsiveness to Immunotherapy in Lung Adenocarcinoma." Biology 2023, 12(6), 808.
4. Junxing Zhao, Zhichao Tang, et al. "Cellular Target Deconvolution of Small Molecules Using a Selection-Based Genetic Screening Platform." ACS Cent Sci. 2022 Oct 26;8(10):1424-1434. PMID: 36313155
5. Appolinaire A Olou, Joe Ambrose, et al. "SHP2 regulates adipose maintenance and adipocyte-pancreatic cancer cell crosstalk via PDHA1." J Cell Commun Signal. 2022 Sep 8. PMID: 36074246
6. Yang Liu, Libo He, et al. "Pharmacological inhibition of sphingolipid synthesis reduces ferroptosis by stimulating the HIF-1 pathway." iScience. 2022 Jun 6;25(7):104533. PMID: 35784791
7. Ziming Li, Tengfei Zhao, et al. "A reactive oxygen species-responsive hydrogel encapsulated with bone marrow derived stem cells promotes repair and regeneration of spinal cord injury." Bioact Mater. 2022 May 9;19:550-568. PMID: 35600969
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Description

Quantitative PCR (qPCR, also called Real-time PCR), is a popular technology for precise analysis of gene expression. It can be classified into two categories according to different methods, dye-based and probe-based, in which, dye-based method is more popular, convenient and less costly. Dye-based qPCR monitors real-time fluorescence of the dye binding to the double-stranded DNA to measure DNA amplification indirectly during each cycle. At a point where the fluorescence signal is distinctly detected over the background, Ct value (Cq value) can be determined. The obtained Ct values can be used to evaluate relative target abundance, or calculate absolute target quantities in reference to an appropriate standard curve. Our product HotStart™ 2X Green qPCR Master Mix provides superior specificity, robust amplification efficiency, ideal reproducibility and stability in quantifying target DNA or cDNA. It’s a 2X PreMix, taking advantage of a hot-start Taq polymerase combined with the antibody. Ideal Taq polymerase and suitable buffer will guarantee preferable specificity and high amplification speed. Green I in the mix which is a DNA intercalator will emit fluorescence when bounds to the double-stranded DNA amplified in each cycle and monitoring the fluorescence by the instrument allows for the indirect quantification of amplification products at real time. This reagent is supplied together with two ROX Reference Dye of different concentration which should be used to normalize the fluorescent signal intensity between reactions for instruments with the corresponding requirements. When conducting the experiment, use the SYBR® or SYBR/FAM scan mode setting on the real-time instrument.

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Protocol

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Components and Storage

Components	5mL 1000 rxn with 10μL reaction 500 rxn with 20μL reaction 200 rxn with 50μL reaction	25mL 5000 rxn with 10μL reaction 2500 rxn with 20μL reaction 1000 rxn with 50μL reaction	50mL 10000 rxn with 10μL reaction 5000 rxn with 20μL reaction 2000 rxn with 50μL reaction
HotStart™ 2X Green qPCR Master Mix	1 mL x 5	1 mL x 25	5 mL x 10
50X ROX Reference Dye (low concentration)	0.2 mL	1 mL	1 mL x 2
50X ROX Reference Dye (high concentration)	0.2 mL	1 mL	1 mL x 2
Store all components at -20°C and protect from light. Avoid repeated freeze/thaw cycles as possible.

Features

Hot-start mechanism for optimized specificity

Superior sensitivity and accuracy

Highly reproducible Ct values over a broad dynamic range

Convenient 2X PreMix formats to simplify experimental steps

Storage

Store all components at -20°C and protect from light. Avoid repeated freeze/thaw cycles as possible.

HotStart™ 2X Green qPCR Master Mix

Cat# K1070-5ml

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Brand : ApexBio Technology

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Product Citation

Description

Quality Control

Related Biological Data

Related Biological Data

Related Biological Data

Components and Storage

Features

Storage

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