GOT1 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommended Dilution	Western blot: 1/1,000-1/5,000. ELISA: 1/10,000-1/40,000. This antibody has been assayed against 1.0 µg of aspartate transaminase [porcine heart] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
Reactivities	Porcine
Host	Sheep
Isotype	IgG
Clonality	Polyclonal
Immunogen	Aspartate aminotransferase / GOT1 from porcine heart
Specificity	This antibody detects porcine GOT1. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 [porcine heart].
Formulation	0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P00503
Background	Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
Synonyms	Aspartate aminotransferase, Transaminase A
Note	Aspartate aminotransferase (AST) is also referred to as glutamic oxaloacetic transaminase (GOT).
Reference Data