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> Anti-GOAT IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated

Anti-GOAT IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated

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Cat# 605-4302

Size : 2mg

Brand : Rockland Immunochemicals

Contact local distributor :


Phone : +1 850 650 7790

Background

Anti-Goat IgG Peroxidase Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Expand Background

Specifications for Goat IgG (H&L) Secondary Antibody Peroxidase Conjugated

Product Details

Rabbit Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated - 605-4302
rabbit anti-Goat IgG Peroxidase Conjugated Antibody, rabbit anti-Goat IgG HRP Conjugated Antibody, HRP anti-goat IgG, Peroxidase anti-Goat IgG
Rabbit
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Goat
Goat IgG whole molecule
Rabbit Anti-Goat IgG secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.

Application Details

ELISA
IHC, WB  - View References
Anti-Goat IgG Peroxidase conjugate has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Formulation

Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store Secondary Antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

References (21)

(). Fatty acid-binding protein 7 triggers α-synuclein oligomerization in glial cells and oligodendrocytes associated with oxidative stress. Acta Pharmacol Sin.
Applications
Undefined
PubMed
(). A novel fatty acid-binding protein 5 and 7 inhibitor ameliorates oligodendrocyte injury in multiple sclerosis mouse models. EBioMedicine
Applications
WB, IB, PCA
PubMed
(). Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes. Biomedicines.
Applications
WB, IB, PCA
PubMed
(). A C. elegans Zona Pellucida domain protein functions via its ZPc domain. PLoS Genet.
Applications
WB, IB, PCA
PubMed
(). Nuclear lamin B1 interactions with chromatin during the circadian cycle are uncoupled from periodic gene expression. Front Genet.
Applications
WB, IB, PCA
PubMed
(). Urinary IgG4 and Smad1 are specific biomarkers for renal structural and functional changes in early stages of diabetic nephropathy. Diabetes.
Applications
E, EIA
PubMed
(). Toxoplasma ubiquitin-like protease 1, a key enzyme in sumoylation and desumoylation pathways, is under the control of non-coding RNAs. Int J Parasitol.
Applications
WB, IB, PCA
PubMed
(). LXRα Regulates Hepatic ChREBPα Activity and Lipogenesis upon Glucose, but Not Fructose Feeding in Mice. Nutrients
Applications
WB, IB, PCA
PubMed
(). Efficient antibody production in the methylotrophic yeast Ogataea minuta by overexpression of chaperones. J Biosci Bioeng.
Applications
WB, IB, PCA
PubMed
(). Cysteine deprivation prevents induction of peroxisome proliferator-activated receptor gamma-2 and adipose differentiation of 3T3-L1 cells. Biochim Biophys Acta Mol Cell Biol Lipids.
Applications
WB, IB, PCA
PubMed
(). A coiled-coil strategy for the directional display of multiple proteins on the surface of iron oxide nanoparticles. Royal Society of Chemisty.
Applications
WB, IB, PCA
Source
(). Integrity of Narrow Epithelial Tubes in the C. elegans Excretory System Requires a Transient Luminal Matrix. PLOS Genetics
Applications
WB, IB, PCA
PubMed
(). Galectin-1 suppression delineates a new strategy to inhibit myeloma-induced angiogenesis and tumoral growth in vivo. Leukemia.
Applications
WB, IB, PCA
PubMed
(). Liver X receptor regulates hepatic nuclear O-GlcNAc signaling and carbohydrate responsive element-binding protein activity. Journal of Lipid Research
Applications
WB, IB, PCA
PubMed
(). Gene expression levels of Casein kinase 1 (CK1) isoforms are correlated to adiponectin levels in adipose tissue of morbid obese patients and site-specific phosphorylation mediated by CK1 influences multimerization of adiponectin. Mol Cell Endocrinol.
Applications
IHC, ICC, Histology
PubMed
(). A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells. Stem Cell Research & Therapy
Applications
WB, IB, PCA
PubMed
(). Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules. Gene.
Applications
WB, IB, PCA
PubMed
(). Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. Journal of Proteomics
Applications
WB, IB, PCA
PubMed
(). CK1δ kinase activity is modulated by Chk1-mediated phosphorylation. PLoS One
Applications
WB, IB, PCA
PubMed
(). Identification of antibody-interacting proteins that contribute to the production of recombinant antibody in mammalian cells. Appl Microbiol Biotechnol.
Applications
WB, IB, PCA
PubMed
(). Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord. BMC Biotechnol.
Applications
IHC, ICC, Histology
PubMed

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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Related Resources

Tips for Selecting the Best Secondary Antibody

Technical Tips
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