Goat anti-VE-cadherin

Print

Cat# EB12667

Size : 100ug

Contact local distributor :

Phone : +1 850 650 7790

Brand : Everest Biotech

Contact local distributor :

Phone : +1 850 650 7790

Code	Name	Applications	Availability	Size	Tested species	Grade
EB12667	Goat Anti-VE-cadherin Antibody	Pep-ELISA, IF, FC		100µg specific antibody in 200µl	Human

EB12667 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

EB12667 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Target Protein

Principal Names: CDH5, cadherin 5, type 2 (vascular endothelium), 7B4, CD144, 7B4 antigen, VE-cadherin, cadherin 5, type 2, VE-cadherin (vascular epithelium), cadherin-5, cd144 antigen, endothelial-specific cadherin, vascular endothelial cadherin
Official Symbol: CDH5
Accession Number(s): NP_001786.2
Human GeneID(s): 1003

Immunogen

Peptide with sequence C-KQARAHGKSVPE, from the internal region of the protein sequence according to NP_001786.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig, Cow