Goat anti-OX1R and OX2R
Cat# EB08608
Size : 100ug
Brand : Everest Biotech
Contact local distributor :
Phone : +1 850 650 7790
| Code | Name | Applications | Availability | Size | Tested species | Grade |
|---|---|---|---|---|---|---|
| EB08608 | Goat Anti-OX1R and OX2R Antibody | Pep-ELISA, WB | 100µg specific antibody in 200µl | Human, Mouse, Rat |
EB08608 (0.5µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08608 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Target Protein
Principal Names: OX1R, OX2R, HCRTR1, hypocretin (orexin) receptor 1, hypocretin receptor 1, hypocretin receptor-1, orexin receptor 1, orexin receptor-1, HCRTR2, hypocretin (orexin) receptor 2, hypocretin receptor 2, hypocretin receptor-2, orexin receptor 2, orexin receptor-2Official Symbol: HCRTR1
Accession Number(s): NP_001516.2; NP_001517.2
Human GeneID(s): 3061 , 3062
Non-Human GeneID(s): 230777 (mouse), 25593 (rat)
Important Comments: This antibody is expected to recognise both the human proteins OX1R and OX2R.
Immunogen
Peptide with sequence C-YNFLSGKFREQFK, from the internal region of the protein sequence according to NP_001516.2; NP_001517.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 48kDa band observed in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates (calculated MW of 47.5kDa according to NP_001516.1 and NP_001517.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol
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