Goat anti-Doublecortin / DCX (aa232-242)
Cat# EB10781
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB10781 | Goat Anti-Doublecortin / DCX (aa232-242) Antibody | Pep-ELISA, IF, IHC | 100µg specific antibody in 200µl | Human, Rat |
Target Protein
Principal Names: DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortinOfficial Symbol: DCX
Accession Number(s): NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1
Human GeneID(s): 1641
Non-Human GeneID(s): 13193 (mouse), 84394 (rat)
Important Comments: This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Immunogen
Peptide with sequence C-KTSANMKAPQS, from the internal region of the protein sequence according to NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5-6µg/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and KNRK cells. Recommended concentration: 10µg/ml.
Species Reactivity
Tested: Human, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Specific Reference
This antibody (previous batch) has been successfully used in IHC on Rat:
Heysieattalab, S., Sadeghi
Dynamic structural neuroplasticity during and after epileptogenesis in a pilocarpine rat model of epilepsy
Acta Epileptologica 3, 3 (2021). https://doi.org/10.1186/s42494-020-00037-7
PMID: 0
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol