Reagents and instruments for immunology, cell biology and molecular biology.
 
> Goat anti-CRHR1 / CRF-R (aa 250 - 263)

Goat anti-CRHR1 / CRF-R (aa 250 - 263)

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Cat# EB07553

Size : 100ug

Brand : Everest Biotech

Contact local distributor :


Phone : +1 850 650 7790

Code Name Applications Availability Size Tested species Grade
EB07553 Goat Anti-CRHR1 / CRF-R (aa 250 - 263) Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse
EB07553 1
EB07553 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07553 2
EB07553 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Target Protein

Principal Names: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor
Official Symbol: CRHR1
Accession Number(s): NP_004373.2
Human GeneID(s): 1394
Non-Human GeneID(s): 12921 (mouse), 58959 (rat)

Immunogen

Peptide with sequence KLYYDNEKCWFGKR, from the internal region of the protein sequence according to NP_004373.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: This antibody has been successfully used in WB on Mouse: Hunt AJ Jr et al, Brain Struct Funct. 2018 Jul;223(6):2685-2698. PMID: 29569009.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Immunofluorescence: Expression of the protein seen in the cytoplasm and vesicles of MCF7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep

Specific References

This antibody has been successfully used on Rat:
Ying Chen , Yan Zhao, Dan-ni Luo , Hui Zheng , Ying Li , and Si-yuan Zhou
Electroacupuncture Regulates Disorders of Gut-Brain Interaction by Decreasing Corticotropin-Releasing Factor in a Rat Model of IBS
Gastroenterology Research and Practice Volume 2019, Article ID 1759842
PMID: 31737064

This antibody has been successfully used in Western blot on Mouse:
Hunt AJ Jr, Dasgupta R, Rajamanickam S, Jiang Z, Beierlein M, Chan CS, Justice NJ
Paraventricular hypothalamic and amygdalar CRF neurons synapse in the external globus pallidus.
Brain Struct Funct. 2018 Jul;223(6):2685-2698.
PMID: 29569009

This antibody (previous batch) has been successfully used in IF on Mouse:
Jiang Z, Rajamanickam S, Justice NJ.
Local Corticotropin-Releasing Factor Signaling in the Hypothalamic Paraventricular Nucleus.
J Neurosci. 2018 Feb 21;38(8):1874-1890.
PMID: 29352046

This antibody (previous batch) has been successfully used in ICC on Rat:
Adrienne L. Andres, Limor Regev, Lucas Phi, Ronald R. Seese, Yuncai Chen, Christine M. Gall and Tallie Z. Baram
NMDA Receptor Activation and Calpain Contribute to Disruption of Dendritic Spines by the Stress Neuropeptide CRH
J Neurosci. 2013 Oct 23;33(43):16945-60.
PMID: 24155300

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