Specifications

Product Data
Applications	ELISA, IP, WB
Recommended Dilution	ELISA. Western blot: 1/5,000. Immunoprecipitation: 1/200-1/500. The antibody has successfully been used in Immunohistochemistry on Vibratome sections and in Filter Binding Assays.
Reactivities	A. victoria
Host	Rabbit
Clonality	Polyclonal
Immunogen	E.coli expressed full-length GFP (Green Fluorescent Protein).
Specificity	This antibody reacts with wild-type GFP, and its variants such as EGFP and EBFP.
Formulation	State: Purified State: Lyophilized purified IgG fraction Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore to 1 mg/ml by adding 0.2 ml water.
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage	Store the antibody undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch
Background	Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA1 and demonstration that GFP can be expressed as a functional transgene2, GFP has become a new tool with exciting applications in developmental, cell and molecular biology3. GFP is an ideal fluorescent probe: its fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells; it can fuse with proteins of interest without interfering significantly with their assembly or function.
Synonyms	Green fluorescent protein, GFP-Tag
Note	This product was originally produced by Torrey Pine Biolabs
Reference Data