GDNF Receptor alpha 1 (GFRA1) (alpha) (24-440) Mouse Monoclonal Antibody [Clone ID: 3H8]
CAT#: AM26645AF-N
GDNF Receptor alpha 1 (GFRA1) (alpha) (24-440) mouse monoclonal antibody, clone 3H8, Azide Free
Specifications
Product Data | |
Clone Name | 3H8 |
Applications | FC, IP |
Recommended Dilution | Immunoprecipitation: 1 μg/100 μl of cell extract from 2 x 10e5 cells. Flow cytometry: 10 μg/ml (final concentration). For details see protocols below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Recombinant human GFRα1 extracellular domain (24-440 aa) |
Specificity | This antibody detects GFRα1. reacts with HCC-1500, MCF-7, but NOT with BT-20, ZR-75-1. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | GDNF family receptor alpha 1 |
Database Link | |
Background | Glial cell line-derived neurotrophic factor (GDNF) family plays a critical role in neurodevelopment and survival of central and peripheral neurons. The biological activity of GDNF family through receptor protein tyrosine kinase Ret and require a ligand specific co-receptor a (GFRa1, binds GDNF, whereas GFRa2 binds NRTN, GFRa3 binds ARTN and GFRa4 binds PSPN). Human GFRa1 is constituted from 465 amino acids and attach to the cell membrane by glycosylphosphatidylinositol (GPI) anchoring manner. It is widely accepted that homodimeric GDNF induce the complex formation of GFRa1 and further the stoichiometry of the signaling complex with GDNF2-GFRa12-Ret2, but the mechanism of complex formation remain unclear. |
Synonyms | GFR-alpha-1, GDNF receptor alpha, RET ligand 1, GDNFRA, RETL1, TRNR1 |
Note | This product was originally produced by MBL International. Protocol: Immunoprecipitation Flow cytometric analysis for adherent cells |
Reference Data |
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