FlyCut^® XhoI is expressed and purified from E.coli that carries the recombinant XhoI gene. The molecular weight is 27.9 kDa, with the recognition site at C^TCGAG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...C↓TCGAG...3’
3’...GAGCT↑C...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)