FlyCut^® NcoI is expressed and purified from E.coli that carries the recombinant NcoI gene. The molecular weight is 32.3 kDa, with the recognition site at C^CATGG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...C↓CATGG...3’
3’...GGTAC↑C...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)