FGF4 Chemi-Luminescent ELISA Kit (Chicken)
Cat# OKCD03481
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for FGF4 Chemi-Luminescent ELISA Kit (Chicken) (OKCD03481) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 4 (FGF4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 4 (FGF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 4 (FGF4) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 1.37-1,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 15.63 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Fgf4 |
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| Alias Symbols | Heparin-binding growth factor 4. |
| NCBI Gene Id | 428857 |
| Protein Name | Fibroblast growth factor 4 |
| Description of Target | Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb development during embryogenesis (By similarity). |
| Uniprot ID | P48804 |
| Protein Accession # | NP_001026717 |
| Nucleotide Accession # | NM_001031546 |